Comparing C60+ and (H2O)n+ clusters for mouse brain tissue analysis

Irma Berrueta Razo; Sadia Sheraz; Alex Henderson; Nicholas P. Lockyer; John C. Vickerman

doi:10.1002/sia.5597

Comparing C60+ and (H2O)n+ clusters for mouse brain tissue analysis

Irma Berrueta Razo, Sadia Sheraz, Alex Henderson, Nicholas P. Lockyer, John C. Vickerman

Research output: Contribution to journal › Article › peer-review

Abstract

Time-of-flight SIMS is applied to the analysis of single cells and different types of biological tissue samples enabling the generation of images with high spatial resolution and chemical specificity. However, the low yield of secondary ions from this type of sample still remains a challenge. This low yield could potentially be increased by enhancing the protonation of ions with the presence of water. Here, we have explored the application of a prototype water cluster ion beam for the analysis of mouse brain tissue samples. A series of experiments acquired with 20keV (H2O)3000+ and 20keV (H2O)4500+ were compared with 20keV C60+, showing ion yield enhancement when a (H2O)n+ cluster ion is employed in the analysis. The results have demonstrated the potential benefits provided by the use of (H2O)n+ clusters for the analysis of mouse brain tissue samples. © 2014 The Authors Surface and Interface Analysis Published by John Wiley & Sons Ltd.

Original language	English
Pages (from-to)	136–139
Number of pages	4
Journal	Surface and Interface Analysis
Volume	46
Issue number	S1
Early online date	25 Nov 2014
DOIs	https://doi.org/10.1002/sia.5597
Publication status	Published - Nov 2014

Keywords

C60
Cluster comparison
H2O
Imaging
SIMS
Tissue analysis

Research Beacons, Institutes and Platforms

Manchester Institute of Biotechnology

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10.1002/sia.5597

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title = "Comparing C60+ and (H2O)n+ clusters for mouse brain tissue analysis",

abstract = "Time-of-flight SIMS is applied to the analysis of single cells and different types of biological tissue samples enabling the generation of images with high spatial resolution and chemical specificity. However, the low yield of secondary ions from this type of sample still remains a challenge. This low yield could potentially be increased by enhancing the protonation of ions with the presence of water. Here, we have explored the application of a prototype water cluster ion beam for the analysis of mouse brain tissue samples. A series of experiments acquired with 20keV (H2O)3000+ and 20keV (H2O)4500+ were compared with 20keV C60+, showing ion yield enhancement when a (H2O)n+ cluster ion is employed in the analysis. The results have demonstrated the potential benefits provided by the use of (H2O)n+ clusters for the analysis of mouse brain tissue samples. {\textcopyright} 2014 The Authors Surface and Interface Analysis Published by John Wiley & Sons Ltd.",

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AU - Berrueta Razo, Irma

AU - Sheraz, Sadia

AU - Henderson, Alex

AU - Lockyer, Nicholas P.

AU - Vickerman, John C.

PY - 2014/11

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N2 - Time-of-flight SIMS is applied to the analysis of single cells and different types of biological tissue samples enabling the generation of images with high spatial resolution and chemical specificity. However, the low yield of secondary ions from this type of sample still remains a challenge. This low yield could potentially be increased by enhancing the protonation of ions with the presence of water. Here, we have explored the application of a prototype water cluster ion beam for the analysis of mouse brain tissue samples. A series of experiments acquired with 20keV (H2O)3000+ and 20keV (H2O)4500+ were compared with 20keV C60+, showing ion yield enhancement when a (H2O)n+ cluster ion is employed in the analysis. The results have demonstrated the potential benefits provided by the use of (H2O)n+ clusters for the analysis of mouse brain tissue samples. © 2014 The Authors Surface and Interface Analysis Published by John Wiley & Sons Ltd.

AB - Time-of-flight SIMS is applied to the analysis of single cells and different types of biological tissue samples enabling the generation of images with high spatial resolution and chemical specificity. However, the low yield of secondary ions from this type of sample still remains a challenge. This low yield could potentially be increased by enhancing the protonation of ions with the presence of water. Here, we have explored the application of a prototype water cluster ion beam for the analysis of mouse brain tissue samples. A series of experiments acquired with 20keV (H2O)3000+ and 20keV (H2O)4500+ were compared with 20keV C60+, showing ion yield enhancement when a (H2O)n+ cluster ion is employed in the analysis. The results have demonstrated the potential benefits provided by the use of (H2O)n+ clusters for the analysis of mouse brain tissue samples. © 2014 The Authors Surface and Interface Analysis Published by John Wiley & Sons Ltd.

KW - C60

KW - Cluster comparison

KW - H2O

KW - Imaging

KW - SIMS

KW - Tissue analysis

U2 - 10.1002/sia.5597

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ER -

Comparing C60+ and (H2O)n+ clusters for mouse brain tissue analysis

Abstract

Keywords

Research Beacons, Institutes and Platforms

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