Comparison of changes in endothelial adhesion molecule expression following UVB irradiation of skin and a human dermal microvascular cell line (HMEC-1)

Lesley Rhodes, Lesley E. Rhodes, M. Joyce, D. C. West, I. Strickland, P. S. Friedmann

    Research output: Contribution to journalArticlepeer-review

    Abstract

    We have assessed the pattern of dermal endothelial adhesion molecule expression following broadband UVB irradiation in vivo and in vitro. Skin biopsies were taken from 4 human volunteers at baseline and at 4, 8 and 24 h post-irradiation with 2.5 minimal erythema doses of UVB. Sections were stained immunohistochemically for E-selectin, intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), CD31 and neutrophil elastase. The effect of direct UVB irradiation on E-selectin, ICAM-1 and VCAM-1 was examined in a human dermal microvascular endothelial cell line, HMEC-1. Cultured HMEC-1 were irradiated with 2.5-40 mJ/cm2 of UVB, and assessed for adhesion molecule expression by immunofluorescence microscopy and fluorescence-activated cell sorter analysis. In vivo, E-selectin was minimally expressed on EC at baseline and was induced by 4 h following irradiation, P
    Original languageEnglish
    Pages (from-to)114-121
    Number of pages7
    JournalPhotodermatology Photoimmunology and Photomedicine
    Volume12
    Issue number3
    Publication statusPublished - 1996

    Keywords

    • adhesion molecules
    • endothelial cells
    • UVB

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