TY - JOUR
T1 - Comparison of four nasal sampling methods for the detection of viral pathogens by RT-PCR-A GA2LEN project
AU - Spyridaki, Irini S.
AU - Christodoulou, Ioannis
AU - de Beer, Lieke
AU - Hovland, Vegard
AU - Kurowski, Marcin
AU - Olszewska-Ziaber, Agnieszka
AU - Carlsen, Kai Håkon
AU - Lødrup-Carlsen, Karin
AU - van Drunen, Cornelis M.
AU - Kowalski, Marek L.
AU - Molenkamp, Richard
AU - Papadopoulos, Nikolaos G.
PY - 2009/3
Y1 - 2009/3
N2 - The aim of this study was to compare the efficacy and patient discomfort between four techniques for obtaining nasal secretions. Nasal secretions from 58 patients with symptoms of a common cold, from three clinical centers (Amsterdam, Lodz, Oslo), were obtained by four different methods: swab, aspirate, brush, and wash. In each patient all four sampling procedures were performed and patient discomfort was evaluated by a visual discomfort scale (scale 1-5) after each procedure. Single pathogen RT-PCRs for Rhinovirus (RV), Influenza virus and Adenovirus, and multiplex real-time PCR for RV, Enterovirus, Influenza virus, Adenovirus, Respiratory Syncytial Virus (RSV), Parainfluenza virus, Coronavirus, Metapneumovirus, Bocavirus and Parechovirus were performed in all samples. A specific viral cause of respiratory tract infection was determined in 48 patients (83%). In these, the detection rate for any virus was 88% (wash), 79% (aspirate), 77% (swab) and 74% (brush). The degree of discomfort reported was 2.54 for swabs, 2.63 for washes, 2.68 for aspirates and 3.61 for brushings. Nasal washes yielded the highest rate of viral detection without excessive patient discomfort. In contrast, nasal brushes produced the lowest detection rates and demonstrated the highest level of discomfort. © 2008 Elsevier B.V. All rights reserved.
AB - The aim of this study was to compare the efficacy and patient discomfort between four techniques for obtaining nasal secretions. Nasal secretions from 58 patients with symptoms of a common cold, from three clinical centers (Amsterdam, Lodz, Oslo), were obtained by four different methods: swab, aspirate, brush, and wash. In each patient all four sampling procedures were performed and patient discomfort was evaluated by a visual discomfort scale (scale 1-5) after each procedure. Single pathogen RT-PCRs for Rhinovirus (RV), Influenza virus and Adenovirus, and multiplex real-time PCR for RV, Enterovirus, Influenza virus, Adenovirus, Respiratory Syncytial Virus (RSV), Parainfluenza virus, Coronavirus, Metapneumovirus, Bocavirus and Parechovirus were performed in all samples. A specific viral cause of respiratory tract infection was determined in 48 patients (83%). In these, the detection rate for any virus was 88% (wash), 79% (aspirate), 77% (swab) and 74% (brush). The degree of discomfort reported was 2.54 for swabs, 2.63 for washes, 2.68 for aspirates and 3.61 for brushings. Nasal washes yielded the highest rate of viral detection without excessive patient discomfort. In contrast, nasal brushes produced the lowest detection rates and demonstrated the highest level of discomfort. © 2008 Elsevier B.V. All rights reserved.
KW - Nasal aspirate
KW - Nasal brush
KW - Nasal swab
KW - Nasal wash
KW - RT-PCR
KW - Upper respiratory infection
U2 - 10.1016/j.jviromet.2008.10.027
DO - 10.1016/j.jviromet.2008.10.027
M3 - Article
C2 - 19041346
SN - 0166-0934
VL - 156
SP - 102
EP - 106
JO - Journal of Virological Methods
JF - Journal of Virological Methods
IS - 1-2
ER -
