Comparison of populations of mould species in homes in the UK and USA using mould-specific quantitative PCR

Stephen J. Vesper, L. J. Wymer, T. Meklin, M. Varma, R. Stott, M. Richardson, R. A. Haugland

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Aims: To compare the populations of 81 mould species in homes in the USA and UK using mould-specific quantitative polymerase chain reaction (MSQPCR) technology. Methods and Results: Dust samples were obtained from randomly selected homes in the UK (n = 11). The mould populations in British homes were compared with those found in typical homes (no visible mould) in the USA (in the state of Ohio, n = 45). Only 13 of 81 species screened showed significantly different concentrations in these two sets of home. Conclusions: Although only a small survey, the results suggest that typical mould profiles in the USA (Ohio) and British homes are very similar. Analysis of 26 mould indicator species revealed that the British homes fell into two clusters, tentatively identified as 'atypical' and 'typical' mould conditions. Significance and Impact of the Study: MSQPCR analysis of dust samples can provide an objective measure of indoor moulds which could lead to better management of their health effects. © 2005 The Society for Applied Microbiology.
    Original languageEnglish
    Pages (from-to)367-373
    Number of pages6
    JournalLetters in Applied Microbiology
    Volume41
    Issue number4
    DOIs
    Publication statusPublished - 2005

    Keywords

    • British
    • Indicator
    • Indoor moulds
    • Mould-specific quantitative PCR
    • Population

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