TY - JOUR
T1 - Comparison of saccharides as osmotic impermeants during hypothermic lung graft preservation
AU - Hopkinson, David N.
AU - Odom, Nicholas J.
AU - Bridgewater, Benjamin J M
AU - Hooper, Timothy I.
PY - 1996/6/27
Y1 - 1996/6/27
N2 - We have previously shown that the trisaccharide raffinose is largely responsible for the superior lung graft performance seen after storage in University of Wisconsin solution. To investigate the use of osmotic agents in perfusates for hypothermic lung graft storage, we compared saccharides of various molecular weights in an isolated rat lung model. Grafts were flushed with 1 of 6 preservation solutions (n=5 each group) containing either a monosaccharide (glucose [G] or fructose [F]), disaccharide (trehalose [T] or sucrose [S]), or trisaccharide (raffinose [R] or melezitose [M]). Grafts were stored for 6 hours at 4°C, reperfused by a veno-venous circuit from an anesthetized support animal for 60 min, and ventilated with room air. The best graft function was seen when trisaccharides were used (PO2; R 126±3 mm Hg, M 129±3 mm Hg; blood flows: R 10.2±0.42 ml/min, M 10.3±0.22 ml/min). Disaccharides produced similar oxygenation (T 133±3 mm Hg, S 129±3 mm Hg) and flows (T 10.3+0.29 ml/min, S 9.7+0.4 ml/min) at 60 min, but initial flows were reduced. Monosaccharides produced the least satisfactory graft function, with impaired oxygenation (F 110±14 mm Hg, P
AB - We have previously shown that the trisaccharide raffinose is largely responsible for the superior lung graft performance seen after storage in University of Wisconsin solution. To investigate the use of osmotic agents in perfusates for hypothermic lung graft storage, we compared saccharides of various molecular weights in an isolated rat lung model. Grafts were flushed with 1 of 6 preservation solutions (n=5 each group) containing either a monosaccharide (glucose [G] or fructose [F]), disaccharide (trehalose [T] or sucrose [S]), or trisaccharide (raffinose [R] or melezitose [M]). Grafts were stored for 6 hours at 4°C, reperfused by a veno-venous circuit from an anesthetized support animal for 60 min, and ventilated with room air. The best graft function was seen when trisaccharides were used (PO2; R 126±3 mm Hg, M 129±3 mm Hg; blood flows: R 10.2±0.42 ml/min, M 10.3±0.22 ml/min). Disaccharides produced similar oxygenation (T 133±3 mm Hg, S 129±3 mm Hg) and flows (T 10.3+0.29 ml/min, S 9.7+0.4 ml/min) at 60 min, but initial flows were reduced. Monosaccharides produced the least satisfactory graft function, with impaired oxygenation (F 110±14 mm Hg, P
U2 - 10.1097/00007890-199606270-00001
DO - 10.1097/00007890-199606270-00001
M3 - Article
C2 - 8685941
SN - 1534-6080
VL - 61
SP - 1667
EP - 1671
JO - Transplantation
JF - Transplantation
IS - 12
ER -