Complement activation by PEG-functionalized multi-walled carbon nanotubes is independent of PEG molecular mass and surface density

Alina J. Andersen; Barbara Windschiegl; Sibel Ilbasmis-Tamer; Ismail T. Degim; Alan Christy Hunter; Thomas L. Andresen; Seyed Moein Moghimi

doi:10.1016/j.nano.2013.01.011

Complement activation by PEG-functionalized multi-walled carbon nanotubes is independent of PEG molecular mass and surface density

Alina J. Andersen, Barbara Windschiegl, Sibel Ilbasmis-Tamer, Ismail T. Degim, Alan Christy Hunter, Thomas L. Andresen, Seyed Moein Moghimi

Research output: Contribution to journal › Article › peer-review

Abstract

Carboxylated (4%) multi-walled carbon nanotubes were covalently functionalized with poly(ethylene glycol)1000 (PEG1000), PEG1500 and PEG4000 with a PEG loading of approximately 11% in all cases. PEG loading generated non-uniform and heterogeneous higher surface structures and increased nanotube width considerably, but all PEGylated nanotube species activated the complement system in human serum equally. Increased PEG loading, through adsorption of methoxyPEG2000(or 5000)-phospholipid conjugates, generated fewer complement activation products; however, complement activation was never completely eliminated. Our observations address the difficulty in making carbon nanotubes more compatible with innate immunity through covalent PEG functionalization as well as double PEGylation strategies. © 2013 Elsevier Inc.

Original language	English
Pages (from-to)	469-473
Number of pages	4
Journal	Nanomedicine: Nanotechnology, Biology, and Medicine
Volume	9
Issue number	4
DOIs	https://doi.org/10.1016/j.nano.2013.01.011
Publication status	Published - May 2013

Keywords

Atomic force microscope
Carbon nanotubes
Complement system
Innate immunity
Poly(ethylene glycol)

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10.1016/j.nano.2013.01.011

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title = "Complement activation by PEG-functionalized multi-walled carbon nanotubes is independent of PEG molecular mass and surface density",

abstract = "Carboxylated (4%) multi-walled carbon nanotubes were covalently functionalized with poly(ethylene glycol)1000 (PEG1000), PEG1500 and PEG4000 with a PEG loading of approximately 11% in all cases. PEG loading generated non-uniform and heterogeneous higher surface structures and increased nanotube width considerably, but all PEGylated nanotube species activated the complement system in human serum equally. Increased PEG loading, through adsorption of methoxyPEG2000(or 5000)-phospholipid conjugates, generated fewer complement activation products; however, complement activation was never completely eliminated. Our observations address the difficulty in making carbon nanotubes more compatible with innate immunity through covalent PEG functionalization as well as double PEGylation strategies. {\textcopyright} 2013 Elsevier Inc.",

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TY - JOUR

T1 - Complement activation by PEG-functionalized multi-walled carbon nanotubes is independent of PEG molecular mass and surface density

AU - Andersen, Alina J.

AU - Windschiegl, Barbara

AU - Ilbasmis-Tamer, Sibel

AU - Degim, Ismail T.

AU - Hunter, Alan Christy

AU - Andresen, Thomas L.

AU - Moghimi, Seyed Moein

PY - 2013/5

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N2 - Carboxylated (4%) multi-walled carbon nanotubes were covalently functionalized with poly(ethylene glycol)1000 (PEG1000), PEG1500 and PEG4000 with a PEG loading of approximately 11% in all cases. PEG loading generated non-uniform and heterogeneous higher surface structures and increased nanotube width considerably, but all PEGylated nanotube species activated the complement system in human serum equally. Increased PEG loading, through adsorption of methoxyPEG2000(or 5000)-phospholipid conjugates, generated fewer complement activation products; however, complement activation was never completely eliminated. Our observations address the difficulty in making carbon nanotubes more compatible with innate immunity through covalent PEG functionalization as well as double PEGylation strategies. © 2013 Elsevier Inc.

AB - Carboxylated (4%) multi-walled carbon nanotubes were covalently functionalized with poly(ethylene glycol)1000 (PEG1000), PEG1500 and PEG4000 with a PEG loading of approximately 11% in all cases. PEG loading generated non-uniform and heterogeneous higher surface structures and increased nanotube width considerably, but all PEGylated nanotube species activated the complement system in human serum equally. Increased PEG loading, through adsorption of methoxyPEG2000(or 5000)-phospholipid conjugates, generated fewer complement activation products; however, complement activation was never completely eliminated. Our observations address the difficulty in making carbon nanotubes more compatible with innate immunity through covalent PEG functionalization as well as double PEGylation strategies. © 2013 Elsevier Inc.

KW - Atomic force microscope

KW - Carbon nanotubes

KW - Complement system

KW - Innate immunity

KW - Poly(ethylene glycol)

U2 - 10.1016/j.nano.2013.01.011

DO - 10.1016/j.nano.2013.01.011

M3 - Article

SN - 1549-9634

VL - 9

SP - 469

EP - 473

JO - Nanomedicine: Nanotechnology, Biology, and Medicine

JF - Nanomedicine: Nanotechnology, Biology, and Medicine

IS - 4

ER -

Complement activation by PEG-functionalized multi-walled carbon nanotubes is independent of PEG molecular mass and surface density

Abstract

Keywords

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