Concentration of various trace elements in the rat retina and their distribution in different structures

Marta Ugarte; Geoffrey W. Grime; Gillian Lord; Kalotina Geraki; Joanna F. Collingwood; Mary E. Finnegan; Hannah Farnfield; Michael Merchant; Melanie J. Bailey; Neil I. Ward; Peter J. Foster; Paul N. Bishop; Neville N. Osborne; GW Grime; JF Collingwood; ME Finnegan; MJ Bailey; PJ Foster; PN Bishop

doi:10.1039/c2mt20157g

Concentration of various trace elements in the rat retina and their distribution in different structures

Marta Ugarte, Geoffrey W. Grime, Gillian Lord, Kalotina Geraki, Joanna F. Collingwood, Mary E. Finnegan, Hannah Farnfield, Michael Merchant, Melanie J. Bailey, Neil I. Ward, Peter J. Foster, Paul N. Bishop, Neville N. Osborne, GW Grime, JF Collingwood, ME Finnegan, MJ Bailey, PJ Foster, PN Bishop

Research output: Contribution to journal › Article › peer-review

Abstract

Inductively coupled plasma mass spectrometry (ICP-MS) was used to quantify the total amount of trace elements in retina from adult male Sprague-Dawley rats (n = 6). Concentration of trace elements within individual retinal areas in frozen sections of the fellow eye was established with the use of two methodologies: (1) particle-induced X-ray emission (PIXE) in combination with 3D depth profiling with Rutherford backscattering spectrometry (RBS) and (2) synchrotron X-ray fluorescence (SXRF) microscopy. The most abundant metal in the retina was zinc, followed by iron and copper. Nickel, manganese, chromium, cobalt, selenium and cadmium were present in very small amounts. The PIXE and SXRF analysis yielded a non-homogenous pattern distribution of metals in the retina. Relatively high levels of zinc were found in the inner part of the photoreceptor inner segments (RIS)/outer limiting membrane (OLM), inner nuclear layer and plexiform layers. Iron was found to accumulate in the retinal pigment epithelium/choroid layer and RIS/OLM. Copper in turn, was localised primarily in the RIS/OLM and plexiform layers. The trace elements iron, copper, and zinc exist in different amounts and locations in the rat retina. © 2012 The Royal Society of Chemistry.

Original language	English
Pages (from-to)	1245-1254
Number of pages	9
Journal	Metallomics
Volume	4
Issue number	12
DOIs	https://doi.org/10.1039/c2mt20157g
Publication status	Published - Dec 2012

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Ugarte, M., Grime, G. W., Lord, G., Geraki, K., Collingwood, J. F., Finnegan, M. E., Farnfield, H., Merchant, M., Bailey, M. J., Ward, N. I., Foster, P. J., Bishop, P. N., Osborne, N. N., Grime, GW., Collingwood, JF., Finnegan, ME., Bailey, MJ., Foster, PJ., & Bishop, PN. (2012). Concentration of various trace elements in the rat retina and their distribution in different structures. Metallomics, 4(12), 1245-1254. https://doi.org/10.1039/c2mt20157g

@article{3e32c8f49960472c9c4901581f77b123,

title = "Concentration of various trace elements in the rat retina and their distribution in different structures",

abstract = "Inductively coupled plasma mass spectrometry (ICP-MS) was used to quantify the total amount of trace elements in retina from adult male Sprague-Dawley rats (n = 6). Concentration of trace elements within individual retinal areas in frozen sections of the fellow eye was established with the use of two methodologies: (1) particle-induced X-ray emission (PIXE) in combination with 3D depth profiling with Rutherford backscattering spectrometry (RBS) and (2) synchrotron X-ray fluorescence (SXRF) microscopy. The most abundant metal in the retina was zinc, followed by iron and copper. Nickel, manganese, chromium, cobalt, selenium and cadmium were present in very small amounts. The PIXE and SXRF analysis yielded a non-homogenous pattern distribution of metals in the retina. Relatively high levels of zinc were found in the inner part of the photoreceptor inner segments (RIS)/outer limiting membrane (OLM), inner nuclear layer and plexiform layers. Iron was found to accumulate in the retinal pigment epithelium/choroid layer and RIS/OLM. Copper in turn, was localised primarily in the RIS/OLM and plexiform layers. The trace elements iron, copper, and zinc exist in different amounts and locations in the rat retina. {\textcopyright} 2012 The Royal Society of Chemistry.",

author = "Marta Ugarte and Grime, {Geoffrey W.} and Gillian Lord and Kalotina Geraki and Collingwood, {Joanna F.} and Finnegan, {Mary E.} and Hannah Farnfield and Michael Merchant and Bailey, {Melanie J.} and Ward, {Neil I.} and Foster, {Peter J.} and Bishop, {Paul N.} and Osborne, {Neville N.} and GW Grime and JF Collingwood and ME Finnegan and MJ Bailey and PJ Foster and PN Bishop",

year = "2012",

month = dec,

doi = "10.1039/c2mt20157g",

language = "English",

volume = "4",

pages = "1245--1254",

journal = "Metallomics",

issn = "1756-5901",

publisher = "Oxford University Press",

number = "12",

}

Ugarte, M, Grime, GW, Lord, G, Geraki, K, Collingwood, JF, Finnegan, ME, Farnfield, H, Merchant, M, Bailey, MJ, Ward, NI, Foster, PJ, Bishop, PN, Osborne, NN, Grime, GW, Collingwood, JF, Finnegan, ME, Bailey, MJ, Foster, PJ & Bishop, PN 2012, 'Concentration of various trace elements in the rat retina and their distribution in different structures', Metallomics, vol. 4, no. 12, pp. 1245-1254. https://doi.org/10.1039/c2mt20157g

TY - JOUR

T1 - Concentration of various trace elements in the rat retina and their distribution in different structures

AU - Ugarte, Marta

AU - Grime, Geoffrey W.

AU - Lord, Gillian

AU - Geraki, Kalotina

AU - Collingwood, Joanna F.

AU - Finnegan, Mary E.

AU - Farnfield, Hannah

AU - Merchant, Michael

AU - Bailey, Melanie J.

AU - Ward, Neil I.

AU - Foster, Peter J.

AU - Bishop, Paul N.

AU - Osborne, Neville N.

AU - Grime, GW

AU - Collingwood, JF

AU - Finnegan, ME

AU - Bailey, MJ

AU - Foster, PJ

AU - Bishop, PN

PY - 2012/12

Y1 - 2012/12

N2 - Inductively coupled plasma mass spectrometry (ICP-MS) was used to quantify the total amount of trace elements in retina from adult male Sprague-Dawley rats (n = 6). Concentration of trace elements within individual retinal areas in frozen sections of the fellow eye was established with the use of two methodologies: (1) particle-induced X-ray emission (PIXE) in combination with 3D depth profiling with Rutherford backscattering spectrometry (RBS) and (2) synchrotron X-ray fluorescence (SXRF) microscopy. The most abundant metal in the retina was zinc, followed by iron and copper. Nickel, manganese, chromium, cobalt, selenium and cadmium were present in very small amounts. The PIXE and SXRF analysis yielded a non-homogenous pattern distribution of metals in the retina. Relatively high levels of zinc were found in the inner part of the photoreceptor inner segments (RIS)/outer limiting membrane (OLM), inner nuclear layer and plexiform layers. Iron was found to accumulate in the retinal pigment epithelium/choroid layer and RIS/OLM. Copper in turn, was localised primarily in the RIS/OLM and plexiform layers. The trace elements iron, copper, and zinc exist in different amounts and locations in the rat retina. © 2012 The Royal Society of Chemistry.

AB - Inductively coupled plasma mass spectrometry (ICP-MS) was used to quantify the total amount of trace elements in retina from adult male Sprague-Dawley rats (n = 6). Concentration of trace elements within individual retinal areas in frozen sections of the fellow eye was established with the use of two methodologies: (1) particle-induced X-ray emission (PIXE) in combination with 3D depth profiling with Rutherford backscattering spectrometry (RBS) and (2) synchrotron X-ray fluorescence (SXRF) microscopy. The most abundant metal in the retina was zinc, followed by iron and copper. Nickel, manganese, chromium, cobalt, selenium and cadmium were present in very small amounts. The PIXE and SXRF analysis yielded a non-homogenous pattern distribution of metals in the retina. Relatively high levels of zinc were found in the inner part of the photoreceptor inner segments (RIS)/outer limiting membrane (OLM), inner nuclear layer and plexiform layers. Iron was found to accumulate in the retinal pigment epithelium/choroid layer and RIS/OLM. Copper in turn, was localised primarily in the RIS/OLM and plexiform layers. The trace elements iron, copper, and zinc exist in different amounts and locations in the rat retina. © 2012 The Royal Society of Chemistry.

U2 - 10.1039/c2mt20157g

DO - 10.1039/c2mt20157g

M3 - Article

C2 - 23093062

SN - 1756-5901

VL - 4

SP - 1245

EP - 1254

JO - Metallomics

JF - Metallomics

IS - 12

ER -

Concentration of various trace elements in the rat retina and their distribution in different structures

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