Conditional neuronal nitric oxide synthase overexpression impairs myocardial contractility

Natalie Burkard; Adam G. Rokita; Susann G. Kaufmann; Matthias Hallhuber; Rongxue Wu; Kai Hu; Ulrich Hofmann; Andreas Bonz; Stefan Frantz; Elizabeth J. Cartwright; Ludwig Neyses; Lars S. Maier; Sebastian K G Maier; Thomas Renné; Kai Schuh; Oliver Ritter

doi:10.1161/01.RES.0000259042.04576.6a

Conditional neuronal nitric oxide synthase overexpression impairs myocardial contractility

Natalie Burkard, Adam G. Rokita, Susann G. Kaufmann, Matthias Hallhuber, Rongxue Wu, Kai Hu, Ulrich Hofmann, Andreas Bonz, Stefan Frantz, Elizabeth J. Cartwright, Ludwig Neyses, Lars S. Maier, Sebastian K G Maier, Thomas Renné, Kai Schuh, Oliver Ritter

Research output: Contribution to journal › Article › peer-review

Abstract

The role of the neuronal NO synthase (nNOS or NOS1) enzyme in the control of cardiac function still remains unclear. Results from nNOS mice or from pharmacological inhibition of nNOS are contradictory and do not pay tribute to the fact that probably spatial confinement of the nNOS enzyme is of major importance. We hypothesize that the close proximity of nNOS and certain effector molecules like L-type Ca-channels has an impact on myocardial contractility. To test this, we generated a new transgenic mouse model allowing conditional, myocardial specific nNOS overexpression. Western blot analysis of transgenic nNOS overexpression showed a 6-fold increase in nNOS protein expression compared with noninduced littermates (n=12; P

Original language	English
Pages (from-to)	e32-e44
Journal	Circulation research
Volume	100
Issue number	3
DOIs	https://doi.org/10.1161/01.RES.0000259042.04576.6a
Publication status	Published - Feb 2007

Keywords

Conditional overexpression
Contractility
Contraction coupling
Excitation
nNOS

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10.1161/01.RES.0000259042.04576.6a

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Burkard, N., Rokita, A. G., Kaufmann, S. G., Hallhuber, M., Wu, R., Hu, K., Hofmann, U., Bonz, A., Frantz, S., Cartwright, E. J., Neyses, L., Maier, L. S., Maier, S. K. G., Renné, T., Schuh, K., & Ritter, O. (2007). Conditional neuronal nitric oxide synthase overexpression impairs myocardial contractility. Circulation research, 100(3), e32-e44. https://doi.org/10.1161/01.RES.0000259042.04576.6a

@article{c331be227bd04f009313918fd46b3ad3,

title = "Conditional neuronal nitric oxide synthase overexpression impairs myocardial contractility",

abstract = "The role of the neuronal NO synthase (nNOS or NOS1) enzyme in the control of cardiac function still remains unclear. Results from nNOS mice or from pharmacological inhibition of nNOS are contradictory and do not pay tribute to the fact that probably spatial confinement of the nNOS enzyme is of major importance. We hypothesize that the close proximity of nNOS and certain effector molecules like L-type Ca-channels has an impact on myocardial contractility. To test this, we generated a new transgenic mouse model allowing conditional, myocardial specific nNOS overexpression. Western blot analysis of transgenic nNOS overexpression showed a 6-fold increase in nNOS protein expression compared with noninduced littermates (n=12; P",

keywords = "Conditional overexpression, Contractility, Contraction coupling, Excitation, nNOS",

author = "Natalie Burkard and Rokita, {Adam G.} and Kaufmann, {Susann G.} and Matthias Hallhuber and Rongxue Wu and Kai Hu and Ulrich Hofmann and Andreas Bonz and Stefan Frantz and Cartwright, {Elizabeth J.} and Ludwig Neyses and Maier, {Lars S.} and Maier, {Sebastian K G} and Thomas Renn{\'e} and Kai Schuh and Oliver Ritter",

year = "2007",

month = feb,

doi = "10.1161/01.RES.0000259042.04576.6a",

language = "English",

volume = "100",

pages = "e32--e44",

journal = "Circulation research",

issn = "1524-4571",

publisher = "Lippincott Williams & Wilkins",

number = "3",

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Burkard, N, Rokita, AG, Kaufmann, SG, Hallhuber, M, Wu, R, Hu, K, Hofmann, U, Bonz, A, Frantz, S, Cartwright, EJ, Neyses, L, Maier, LS, Maier, SKG, Renné, T, Schuh, K & Ritter, O 2007, 'Conditional neuronal nitric oxide synthase overexpression impairs myocardial contractility', Circulation research, vol. 100, no. 3, pp. e32-e44. https://doi.org/10.1161/01.RES.0000259042.04576.6a

TY - JOUR

T1 - Conditional neuronal nitric oxide synthase overexpression impairs myocardial contractility

AU - Burkard, Natalie

AU - Rokita, Adam G.

AU - Kaufmann, Susann G.

AU - Hallhuber, Matthias

AU - Wu, Rongxue

AU - Hu, Kai

AU - Hofmann, Ulrich

AU - Bonz, Andreas

AU - Frantz, Stefan

AU - Cartwright, Elizabeth J.

AU - Neyses, Ludwig

AU - Maier, Lars S.

AU - Maier, Sebastian K G

AU - Renné, Thomas

AU - Schuh, Kai

AU - Ritter, Oliver

PY - 2007/2

Y1 - 2007/2

N2 - The role of the neuronal NO synthase (nNOS or NOS1) enzyme in the control of cardiac function still remains unclear. Results from nNOS mice or from pharmacological inhibition of nNOS are contradictory and do not pay tribute to the fact that probably spatial confinement of the nNOS enzyme is of major importance. We hypothesize that the close proximity of nNOS and certain effector molecules like L-type Ca-channels has an impact on myocardial contractility. To test this, we generated a new transgenic mouse model allowing conditional, myocardial specific nNOS overexpression. Western blot analysis of transgenic nNOS overexpression showed a 6-fold increase in nNOS protein expression compared with noninduced littermates (n=12; P

AB - The role of the neuronal NO synthase (nNOS or NOS1) enzyme in the control of cardiac function still remains unclear. Results from nNOS mice or from pharmacological inhibition of nNOS are contradictory and do not pay tribute to the fact that probably spatial confinement of the nNOS enzyme is of major importance. We hypothesize that the close proximity of nNOS and certain effector molecules like L-type Ca-channels has an impact on myocardial contractility. To test this, we generated a new transgenic mouse model allowing conditional, myocardial specific nNOS overexpression. Western blot analysis of transgenic nNOS overexpression showed a 6-fold increase in nNOS protein expression compared with noninduced littermates (n=12; P

KW - Conditional overexpression

KW - Contractility

KW - Contraction coupling

KW - Excitation

KW - nNOS

U2 - 10.1161/01.RES.0000259042.04576.6a

DO - 10.1161/01.RES.0000259042.04576.6a

M3 - Article

SN - 1524-4571

VL - 100

SP - e32-e44

JO - Circulation research

JF - Circulation research

IS - 3

ER -

Conditional neuronal nitric oxide synthase overexpression impairs myocardial contractility

Abstract

Keywords

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