Conserved microsatellite markers of high cross-species utility for flying, ground and tree squirrels

Salisa Jumpa; Deborah A Dawson; Gavin J Horsburgh; Catherine Walton

doi:10.1007/s12686-015-0439-1

Conserved microsatellite markers of high cross-species utility for flying, ground and tree squirrels

Salisa Jumpa, Deborah A Dawson, Gavin J Horsburgh, Catherine Walton

Research output: Contribution to journal › Article › peer-review

Abstract

Many squirrel species around the world are threatened by forest loss and fragmentation. To facilitate studies of squirrel biodiversity, particularly of flying squirrels in Southeast Asia, we identified Hylopetes, Menetes, Glaucomys and Sciurus squirrel microsatellite sequences with homologs in a second squirrel species (Spermophilus tridecemlineatus), designed 40 consensus markers and tested three squirrel species. When tested in four individuals per species, 26 markers were variable in Hylopetes phayrei, 25 markers in H. lepidus and 25 markers in Menetes berdmorei. Eleven markers were selected from 14 that were polymorphic in all three species. Cross-species utility was confirmed for these 11 markers in seven additional squirrel species, including: the flying squirrels H. phayrei, H. lepidus, H. spadiceus and Petaurista petaurista; a ground squirrel, M. berdmorei; and the tree squirrels, Callosciurus caniceps and C. finlaysoni. The other markers that were variable in one or multiple species are also useful for those specific species.

Original language	English
Pages (from-to)	599-603
Number of pages	4
Journal	Conservation Genetics Resources
Volume	7
Issue number	2
DOIs	https://doi.org/10.1007/s12686-015-0439-1
Publication status	Published - Jun 2015

Keywords

Enhanced cross-species utility Sciuridae Simple tandem repeat (STR) Squirrel Thailand

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s12686-015-0439-1

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title = "Conserved microsatellite markers of high cross-species utility for flying, ground and tree squirrels",

abstract = "Many squirrel species around the world are threatened by forest loss and fragmentation. To facilitate studies of squirrel biodiversity, particularly of flying squirrels in Southeast Asia, we identified Hylopetes, Menetes, Glaucomys and Sciurus squirrel microsatellite sequences with homologs in a second squirrel species (Spermophilus tridecemlineatus), designed 40 consensus markers and tested three squirrel species. When tested in four individuals per species, 26 markers were variable in Hylopetes phayrei, 25 markers in H. lepidus and 25 markers in Menetes berdmorei. Eleven markers were selected from 14 that were polymorphic in all three species. Cross-species utility was confirmed for these 11 markers in seven additional squirrel species, including: the flying squirrels H. phayrei, H. lepidus, H. spadiceus and Petaurista petaurista; a ground squirrel, M. berdmorei; and the tree squirrels, Callosciurus caniceps and C. finlaysoni. The other markers that were variable in one or multiple species are also useful for those specific species.",

keywords = "Enhanced cross-species utility Sciuridae Simple tandem repeat (STR) Squirrel Thailand",

author = "Salisa Jumpa and Dawson, {Deborah A} and Horsburgh, {Gavin J} and Catherine Walton",

year = "2015",

month = jun,

doi = "10.1007/s12686-015-0439-1",

language = "English",

volume = "7",

pages = "599--603",

journal = "Conservation Genetics Resources",

issn = "1877-7252",

publisher = "Springer Nature",

number = "2",

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TY - JOUR

T1 - Conserved microsatellite markers of high cross-species utility for flying, ground and tree squirrels

AU - Jumpa, Salisa

AU - Dawson, Deborah A

AU - Horsburgh, Gavin J

AU - Walton, Catherine

PY - 2015/6

Y1 - 2015/6

N2 - Many squirrel species around the world are threatened by forest loss and fragmentation. To facilitate studies of squirrel biodiversity, particularly of flying squirrels in Southeast Asia, we identified Hylopetes, Menetes, Glaucomys and Sciurus squirrel microsatellite sequences with homologs in a second squirrel species (Spermophilus tridecemlineatus), designed 40 consensus markers and tested three squirrel species. When tested in four individuals per species, 26 markers were variable in Hylopetes phayrei, 25 markers in H. lepidus and 25 markers in Menetes berdmorei. Eleven markers were selected from 14 that were polymorphic in all three species. Cross-species utility was confirmed for these 11 markers in seven additional squirrel species, including: the flying squirrels H. phayrei, H. lepidus, H. spadiceus and Petaurista petaurista; a ground squirrel, M. berdmorei; and the tree squirrels, Callosciurus caniceps and C. finlaysoni. The other markers that were variable in one or multiple species are also useful for those specific species.

AB - Many squirrel species around the world are threatened by forest loss and fragmentation. To facilitate studies of squirrel biodiversity, particularly of flying squirrels in Southeast Asia, we identified Hylopetes, Menetes, Glaucomys and Sciurus squirrel microsatellite sequences with homologs in a second squirrel species (Spermophilus tridecemlineatus), designed 40 consensus markers and tested three squirrel species. When tested in four individuals per species, 26 markers were variable in Hylopetes phayrei, 25 markers in H. lepidus and 25 markers in Menetes berdmorei. Eleven markers were selected from 14 that were polymorphic in all three species. Cross-species utility was confirmed for these 11 markers in seven additional squirrel species, including: the flying squirrels H. phayrei, H. lepidus, H. spadiceus and Petaurista petaurista; a ground squirrel, M. berdmorei; and the tree squirrels, Callosciurus caniceps and C. finlaysoni. The other markers that were variable in one or multiple species are also useful for those specific species.

KW - Enhanced cross-species utility Sciuridae Simple tandem repeat (STR) Squirrel Thailand

U2 - 10.1007/s12686-015-0439-1

DO - 10.1007/s12686-015-0439-1

M3 - Article

SN - 1877-7252

VL - 7

SP - 599

EP - 603

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

IS - 2

ER -

Conserved microsatellite markers of high cross-species utility for flying, ground and tree squirrels

Abstract

Keywords

UN SDGs

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