Controlled microchannelling in dense collagen scaffolds by soluble phosphate glass fibers

Showan N. Nazhat; Ensanya A. Abou Neel; Asmeret Kidane; Iffy Ahmed; Chirs Hope; Matt Kershaw; Peter D. Lee; Eleanor Stride; Nader Saffari; Jonathan C. Knowles; Robert A. Brown

doi:10.1021/bm060715f

Controlled microchannelling in dense collagen scaffolds by soluble phosphate glass fibers

Showan N. Nazhat, Ensanya A. Abou Neel, Asmeret Kidane, Iffy Ahmed, Chirs Hope, Matt Kershaw, Peter D. Lee, Eleanor Stride, Nader Saffari, Jonathan C. Knowles, Robert A. Brown

Research output: Contribution to journal › Article › peer-review

Abstract

A problem with tissue engineering scaffolds is maintaining seeded cell viability and function due to limitations of oxygen and nutrient transfer. An approach to maintain suitable oxygen concentrations throughout the scaffold would be to controllably incorporate microchannelling within these scaffolds. This study investigated the incorporation of unidirectionally aligned soluble phosphate based glass fibers (PGF) into dense collagen scaffolds. PGF are degradable, and their degradation can be controlled through their chemistry and dimensions. Plastic compression was used to produce composite scaffolds at three different weight percentage while maintaining greater than 80% resident cell viability. PGF-collagen scaffold composition was quantified through thermogravimetric analysis as well as being morphologically and mechanically characterized. PGF degradation was measured through ion chromatography, and channel formation was verified with ultrasound imaging and SEM. The free movement of coated microbubble agents confirmed the channels to be continuous in nature and of 30-40 μm diameter. These microchannels in dense native collagen matrices could play an important role in hypoxia/perfusion limitations and also in the transportation of nutrients and potentially forming blood vessels through dense implants. © 2007 American Chemical Society.

Original language	English
Pages (from-to)	543-551
Number of pages	8
Journal	Biomacromolecules
Volume	8
Issue number	2
DOIs	https://doi.org/10.1021/bm060715f
Publication status	Published - Feb 2007

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10.1021/bm060715f

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@article{ae6786bc0a4f4baca64016ffa5bdc95e,

title = "Controlled microchannelling in dense collagen scaffolds by soluble phosphate glass fibers",

abstract = "A problem with tissue engineering scaffolds is maintaining seeded cell viability and function due to limitations of oxygen and nutrient transfer. An approach to maintain suitable oxygen concentrations throughout the scaffold would be to controllably incorporate microchannelling within these scaffolds. This study investigated the incorporation of unidirectionally aligned soluble phosphate based glass fibers (PGF) into dense collagen scaffolds. PGF are degradable, and their degradation can be controlled through their chemistry and dimensions. Plastic compression was used to produce composite scaffolds at three different weight percentage while maintaining greater than 80% resident cell viability. PGF-collagen scaffold composition was quantified through thermogravimetric analysis as well as being morphologically and mechanically characterized. PGF degradation was measured through ion chromatography, and channel formation was verified with ultrasound imaging and SEM. The free movement of coated microbubble agents confirmed the channels to be continuous in nature and of 30-40 μm diameter. These microchannels in dense native collagen matrices could play an important role in hypoxia/perfusion limitations and also in the transportation of nutrients and potentially forming blood vessels through dense implants. {\textcopyright} 2007 American Chemical Society.",

author = "Nazhat, {Showan N.} and {Abou Neel}, {Ensanya A.} and Asmeret Kidane and Iffy Ahmed and Chirs Hope and Matt Kershaw and Lee, {Peter D.} and Eleanor Stride and Nader Saffari and Knowles, {Jonathan C.} and Brown, {Robert A.}",

year = "2007",

month = feb,

doi = "10.1021/bm060715f",

language = "English",

volume = "8",

pages = "543--551",

journal = "Biomacromolecules",

issn = "1526-4602",

publisher = "American Chemical Society",

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T1 - Controlled microchannelling in dense collagen scaffolds by soluble phosphate glass fibers

AU - Nazhat, Showan N.

AU - Abou Neel, Ensanya A.

AU - Kidane, Asmeret

AU - Ahmed, Iffy

AU - Hope, Chirs

AU - Kershaw, Matt

AU - Lee, Peter D.

AU - Stride, Eleanor

AU - Saffari, Nader

AU - Knowles, Jonathan C.

AU - Brown, Robert A.

PY - 2007/2

Y1 - 2007/2

N2 - A problem with tissue engineering scaffolds is maintaining seeded cell viability and function due to limitations of oxygen and nutrient transfer. An approach to maintain suitable oxygen concentrations throughout the scaffold would be to controllably incorporate microchannelling within these scaffolds. This study investigated the incorporation of unidirectionally aligned soluble phosphate based glass fibers (PGF) into dense collagen scaffolds. PGF are degradable, and their degradation can be controlled through their chemistry and dimensions. Plastic compression was used to produce composite scaffolds at three different weight percentage while maintaining greater than 80% resident cell viability. PGF-collagen scaffold composition was quantified through thermogravimetric analysis as well as being morphologically and mechanically characterized. PGF degradation was measured through ion chromatography, and channel formation was verified with ultrasound imaging and SEM. The free movement of coated microbubble agents confirmed the channels to be continuous in nature and of 30-40 μm diameter. These microchannels in dense native collagen matrices could play an important role in hypoxia/perfusion limitations and also in the transportation of nutrients and potentially forming blood vessels through dense implants. © 2007 American Chemical Society.

AB - A problem with tissue engineering scaffolds is maintaining seeded cell viability and function due to limitations of oxygen and nutrient transfer. An approach to maintain suitable oxygen concentrations throughout the scaffold would be to controllably incorporate microchannelling within these scaffolds. This study investigated the incorporation of unidirectionally aligned soluble phosphate based glass fibers (PGF) into dense collagen scaffolds. PGF are degradable, and their degradation can be controlled through their chemistry and dimensions. Plastic compression was used to produce composite scaffolds at three different weight percentage while maintaining greater than 80% resident cell viability. PGF-collagen scaffold composition was quantified through thermogravimetric analysis as well as being morphologically and mechanically characterized. PGF degradation was measured through ion chromatography, and channel formation was verified with ultrasound imaging and SEM. The free movement of coated microbubble agents confirmed the channels to be continuous in nature and of 30-40 μm diameter. These microchannels in dense native collagen matrices could play an important role in hypoxia/perfusion limitations and also in the transportation of nutrients and potentially forming blood vessels through dense implants. © 2007 American Chemical Society.

U2 - 10.1021/bm060715f

DO - 10.1021/bm060715f

M3 - Article

SN - 1526-4602

VL - 8

SP - 543

EP - 551

JO - Biomacromolecules

JF - Biomacromolecules

IS - 2

ER -

Controlled microchannelling in dense collagen scaffolds by soluble phosphate glass fibers

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