Correlation between CD8+ T cells specific for prostate-specific antigen and level of disease in patients with prostate cancer

Eyad Elkord; Anthony W. Rowbottom; Howard Kynaston; Paul E. Williams

doi:10.1016/j.clim.2005.12.008

Correlation between CD8+ T cells specific for prostate-specific antigen and level of disease in patients with prostate cancer

Eyad Elkord, Anthony W. Rowbottom, Howard Kynaston, Paul E. Williams

Research output: Contribution to journal › Article › peer-review

Abstract

Modest work has been performed to improve the sensitivity of residual disease detection or investigate the contribution that the immune system makes in controlling metastatic tumor growth, in particular, the frequency and biological actions of peptide-specific CD8+ T lymphocytes in limiting metastatic disease and/or maintaining remission. Fifty-three peripheral blood samples from 32 prostate cancer (PC) patients were investigated for the presence of circulating prostate-specific antigen (PSA)-expressing cells (CPECs) using a highly sensitive and specific assay combining immunomagnetic epithelial cell enrichment with nested RT-PCR of PSA mRNA. Using HLA-A2 tetramer complexes, frequency of CD8+ T cells specific for PSA-derived peptides was determined. Additionally, serum concentrations of PSA and testosterone were measured. CPECs were detected in 26% of peripheral blood samples from PC patients. CD8+ T cells specific for PSA-derived peptides were detected at low frequency in HLA-A2-positive PC patients. The correlation between these PSA-specific CD8+ T cells and residual prostate tumor cells and clinical measures was investigated. Our data suggest that frequency of PSA-specific CD8+ T cells is correlated to CPECs, but not to serum PSA level. © 2006 Elsevier Inc. All rights reserved.

Original language	English
Pages (from-to)	91-98
Number of pages	7
Journal	Clinical Immunology
Volume	120
Issue number	1
DOIs	https://doi.org/10.1016/j.clim.2005.12.008
Publication status	Published - Jul 2006

Keywords

CD8+ T cells
Circulating PSA-expressing cells (CPECs)
Prostate cancer
Prostate-specific antigen (PSA)
Tetramer

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.clim.2005.12.008

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title = "Correlation between CD8+ T cells specific for prostate-specific antigen and level of disease in patients with prostate cancer",

abstract = "Modest work has been performed to improve the sensitivity of residual disease detection or investigate the contribution that the immune system makes in controlling metastatic tumor growth, in particular, the frequency and biological actions of peptide-specific CD8+ T lymphocytes in limiting metastatic disease and/or maintaining remission. Fifty-three peripheral blood samples from 32 prostate cancer (PC) patients were investigated for the presence of circulating prostate-specific antigen (PSA)-expressing cells (CPECs) using a highly sensitive and specific assay combining immunomagnetic epithelial cell enrichment with nested RT-PCR of PSA mRNA. Using HLA-A2 tetramer complexes, frequency of CD8+ T cells specific for PSA-derived peptides was determined. Additionally, serum concentrations of PSA and testosterone were measured. CPECs were detected in 26\% of peripheral blood samples from PC patients. CD8+ T cells specific for PSA-derived peptides were detected at low frequency in HLA-A2-positive PC patients. The correlation between these PSA-specific CD8+ T cells and residual prostate tumor cells and clinical measures was investigated. Our data suggest that frequency of PSA-specific CD8+ T cells is correlated to CPECs, but not to serum PSA level. {\textcopyright} 2006 Elsevier Inc. All rights reserved.",

keywords = "CD8+ T cells, Circulating PSA-expressing cells (CPECs), Prostate cancer, Prostate-specific antigen (PSA), Tetramer",

author = "Eyad Elkord and Rowbottom, \{Anthony W.\} and Howard Kynaston and Williams, \{Paul E.\}",

year = "2006",

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doi = "10.1016/j.clim.2005.12.008",

language = "English",

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T1 - Correlation between CD8+ T cells specific for prostate-specific antigen and level of disease in patients with prostate cancer

AU - Elkord, Eyad

AU - Rowbottom, Anthony W.

AU - Kynaston, Howard

AU - Williams, Paul E.

PY - 2006/7

Y1 - 2006/7

N2 - Modest work has been performed to improve the sensitivity of residual disease detection or investigate the contribution that the immune system makes in controlling metastatic tumor growth, in particular, the frequency and biological actions of peptide-specific CD8+ T lymphocytes in limiting metastatic disease and/or maintaining remission. Fifty-three peripheral blood samples from 32 prostate cancer (PC) patients were investigated for the presence of circulating prostate-specific antigen (PSA)-expressing cells (CPECs) using a highly sensitive and specific assay combining immunomagnetic epithelial cell enrichment with nested RT-PCR of PSA mRNA. Using HLA-A2 tetramer complexes, frequency of CD8+ T cells specific for PSA-derived peptides was determined. Additionally, serum concentrations of PSA and testosterone were measured. CPECs were detected in 26% of peripheral blood samples from PC patients. CD8+ T cells specific for PSA-derived peptides were detected at low frequency in HLA-A2-positive PC patients. The correlation between these PSA-specific CD8+ T cells and residual prostate tumor cells and clinical measures was investigated. Our data suggest that frequency of PSA-specific CD8+ T cells is correlated to CPECs, but not to serum PSA level. © 2006 Elsevier Inc. All rights reserved.

AB - Modest work has been performed to improve the sensitivity of residual disease detection or investigate the contribution that the immune system makes in controlling metastatic tumor growth, in particular, the frequency and biological actions of peptide-specific CD8+ T lymphocytes in limiting metastatic disease and/or maintaining remission. Fifty-three peripheral blood samples from 32 prostate cancer (PC) patients were investigated for the presence of circulating prostate-specific antigen (PSA)-expressing cells (CPECs) using a highly sensitive and specific assay combining immunomagnetic epithelial cell enrichment with nested RT-PCR of PSA mRNA. Using HLA-A2 tetramer complexes, frequency of CD8+ T cells specific for PSA-derived peptides was determined. Additionally, serum concentrations of PSA and testosterone were measured. CPECs were detected in 26% of peripheral blood samples from PC patients. CD8+ T cells specific for PSA-derived peptides were detected at low frequency in HLA-A2-positive PC patients. The correlation between these PSA-specific CD8+ T cells and residual prostate tumor cells and clinical measures was investigated. Our data suggest that frequency of PSA-specific CD8+ T cells is correlated to CPECs, but not to serum PSA level. © 2006 Elsevier Inc. All rights reserved.

KW - CD8+ T cells

KW - Circulating PSA-expressing cells (CPECs)

KW - Prostate cancer

KW - Prostate-specific antigen (PSA)

KW - Tetramer

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DO - 10.1016/j.clim.2005.12.008

M3 - Article

SN - 1521-6616

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JO - Clinical Immunology

JF - Clinical Immunology

IS - 1

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Correlation between CD8+ T cells specific for prostate-specific antigen and level of disease in patients with prostate cancer

Abstract

Keywords

UN SDGs

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