Projects per year
Upconverting phosphors (UCPs) convert multiple low energy photons into higher energy emission via the process of photon upconversion and offer an attractive alternative to organic fluorophores for use as luminescent probes. Here, UCPs were capped with functionalized silica in order to provide a surface to covalently conjugate proteins with surface-accessible cysteines. Variants of green fluorescent protein (GFP) and the flavoenzyme pentaerythritol tetranitrate reductase (PETNR) were then attached via maleimide-thiol coupling in order to allow energy transfer from the UCP to the GFP or flavin cofactor of PETNR, respectively. PETNR retains its activity when coupled to the UCPs, which allows reversible detection of enzyme substrates via ratiometric sensing of the enzyme redox state.
|Journal||Chemistry – A European Journal|
|Publication status||Published - 31 May 2020|
Research Beacons, Institutes and Platforms
- Photon Science Institute
- Manchester Institute of Biotechnology
FingerprintDive into the research topics of 'Covalent attachment of active enzymes to upconversion phosphors allows ratiometric detection of substrates'. Together they form a unique fingerprint.
- 1 Finished
Linking experiment to theory: Quantum entanglement during enzyme catalysis - Dr S Hay fellowship
1/09/10 → 31/08/15