Creation of protein nanoarrays using Dip-Pen Nanolithography

David G. Thompson, Ekaterina McKenna, Andy Pitt, Duncan Graham

Research output: Chapter in Book/Conference proceedingConference contributionpeer-review

Abstract

Protein microarrays are one of the leading tools for analysing the proteome due to a range of factors including; their relatively simplistic creation and use in an assay, low cost and long shelf life. One of the primary limiting factors in conventional microarray assay systems is that they create a spot of protein ~100-300 μm in diameter. The miniaturisation of these spots will allow for the creation of much denser arrays and hence will increase the number of simultaneous protein detections possible whilst decreasing the overall physical size of the array. One of the methods for creating protein nanoarrays currently showing promise is Dip-Pen Nanolithography (DPN), a scanning probe microscopy technique. This paper outlines the creation of two types of ultra small functional protein microarrays using DPN on a nitrocellulose surface and their use to detect proteinrprotein interaction.

Original languageEnglish
Title of host publicationNanotechnology 2010
Subtitle of host publicationBio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
Pages444-447
Number of pages4
Volume3
Publication statusPublished - 2010
EventNanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 - Anaheim, United States
Duration: 21 Jun 201024 Jun 2010

Publication series

NameNanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
Volume3

Conference

ConferenceNanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
Country/TerritoryUnited States
CityAnaheim
Period21/06/1024/06/10

Keywords

  • Dip-pen nanolithography
  • Protein nanoarrays

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