Crystallization and preliminary crystallographic analysis of a novel cytochrome P450 from Mycobacterium tuberculosis

Christopher G. Mowat; David Leys; Kirsty J. McLean; Stuart L. Rivers; Alison Richmond; Andrew W. Munro; Miguel Ortiz Lombardia; Pedro M. Alzari; Graeme A. Reid; Stephen K. Chapman; Malcolm D. Walkinshaw

doi:10.1107/S0907444902002676

Crystallization and preliminary crystallographic analysis of a novel cytochrome P450 from Mycobacterium tuberculosis

Christopher G. Mowat
, David Leys
, Kirsty J. McLean
, Stuart L. Rivers
, Alison Richmond
, Andrew W. Munro
, Miguel Ortiz Lombardia
, Pedro M. Alzari
, Graeme A. Reid
, Stephen K. Chapman
, Malcolm D. Walkinshaw

Research output: Contribution to journal › Article › peer-review

Abstract

The product of the Rv2276 gene of Mycobacterium tuberculosis is a cytochrome P450 (P450 MT2, CYP121) which has been shown to bind tightly to a range of azole-based antifungal drugs (e.g. miconazole, clotrimazole). These drugs are potent inhibitors of mycobacterial growth, suggesting that P450 MT2 (CYP121) may be a potential drug target. The enzyme has been overexpressed in Escherichia coli and crystallized by the hanging-drop method. Crystals of P450 MT2 (CYP121) belong to the hexagonal space group P6122 or P6522, with unit-cell parameters a = b = 78.3, c = 265.6 Å. Native data have been collected to 1.6 Å resolution and Hg-derivative data to 2.5 Å resolution using a synchrotron-radiation source.

Original language	English
Pages (from-to)	704-705
Number of pages	1
Journal	Acta Crystallographica Section D: Biological Crystallography
Volume	58
Issue number	4
DOIs	https://doi.org/10.1107/S0907444902002676
Publication status	Published - 2002

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Mowat, C. G., Leys, D., McLean, K. J., Rivers, S. L., Richmond, A., Munro, A. W., Ortiz Lombardia, M., Alzari, P. M., Reid, G. A., Chapman, S. K., & Walkinshaw, M. D. (2002). Crystallization and preliminary crystallographic analysis of a novel cytochrome P450 from Mycobacterium tuberculosis. Acta Crystallographica Section D: Biological Crystallography, 58(4), 704-705. https://doi.org/10.1107/S0907444902002676

@article{e5f79a42081b446595e3589d9f6ee3bf,

title = "Crystallization and preliminary crystallographic analysis of a novel cytochrome P450 from Mycobacterium tuberculosis",

abstract = "The product of the Rv2276 gene of Mycobacterium tuberculosis is a cytochrome P450 (P450 MT2, CYP121) which has been shown to bind tightly to a range of azole-based antifungal drugs (e.g. miconazole, clotrimazole). These drugs are potent inhibitors of mycobacterial growth, suggesting that P450 MT2 (CYP121) may be a potential drug target. The enzyme has been overexpressed in Escherichia coli and crystallized by the hanging-drop method. Crystals of P450 MT2 (CYP121) belong to the hexagonal space group P6122 or P6522, with unit-cell parameters a = b = 78.3, c = 265.6 {\AA}. Native data have been collected to 1.6 {\AA} resolution and Hg-derivative data to 2.5 {\AA} resolution using a synchrotron-radiation source.",

author = "Mowat, \{Christopher G.\} and David Leys and McLean, \{Kirsty J.\} and Rivers, \{Stuart L.\} and Alison Richmond and Munro, \{Andrew W.\} and \{Ortiz Lombardia\}, Miguel and Alzari, \{Pedro M.\} and Reid, \{Graeme A.\} and Chapman, \{Stephen K.\} and Walkinshaw, \{Malcolm D.\}",

year = "2002",

doi = "10.1107/S0907444902002676",

language = "English",

volume = "58",

pages = "704--705",

journal = "Acta Crystallographica Section D: Biological Crystallography",

issn = "0907-4449",

publisher = "International Union of Crystallography",

number = "4",

}

Mowat, CG, Leys, D, McLean, KJ, Rivers, SL, Richmond, A, Munro, AW, Ortiz Lombardia, M, Alzari, PM, Reid, GA, Chapman, SK & Walkinshaw, MD 2002, 'Crystallization and preliminary crystallographic analysis of a novel cytochrome P450 from Mycobacterium tuberculosis', Acta Crystallographica Section D: Biological Crystallography, vol. 58, no. 4, pp. 704-705. https://doi.org/10.1107/S0907444902002676

TY - JOUR

T1 - Crystallization and preliminary crystallographic analysis of a novel cytochrome P450 from Mycobacterium tuberculosis

AU - Mowat, Christopher G.

AU - Leys, David

AU - McLean, Kirsty J.

AU - Rivers, Stuart L.

AU - Richmond, Alison

AU - Munro, Andrew W.

AU - Ortiz Lombardia, Miguel

AU - Alzari, Pedro M.

AU - Reid, Graeme A.

AU - Chapman, Stephen K.

AU - Walkinshaw, Malcolm D.

PY - 2002

Y1 - 2002

N2 - The product of the Rv2276 gene of Mycobacterium tuberculosis is a cytochrome P450 (P450 MT2, CYP121) which has been shown to bind tightly to a range of azole-based antifungal drugs (e.g. miconazole, clotrimazole). These drugs are potent inhibitors of mycobacterial growth, suggesting that P450 MT2 (CYP121) may be a potential drug target. The enzyme has been overexpressed in Escherichia coli and crystallized by the hanging-drop method. Crystals of P450 MT2 (CYP121) belong to the hexagonal space group P6122 or P6522, with unit-cell parameters a = b = 78.3, c = 265.6 Å. Native data have been collected to 1.6 Å resolution and Hg-derivative data to 2.5 Å resolution using a synchrotron-radiation source.

AB - The product of the Rv2276 gene of Mycobacterium tuberculosis is a cytochrome P450 (P450 MT2, CYP121) which has been shown to bind tightly to a range of azole-based antifungal drugs (e.g. miconazole, clotrimazole). These drugs are potent inhibitors of mycobacterial growth, suggesting that P450 MT2 (CYP121) may be a potential drug target. The enzyme has been overexpressed in Escherichia coli and crystallized by the hanging-drop method. Crystals of P450 MT2 (CYP121) belong to the hexagonal space group P6122 or P6522, with unit-cell parameters a = b = 78.3, c = 265.6 Å. Native data have been collected to 1.6 Å resolution and Hg-derivative data to 2.5 Å resolution using a synchrotron-radiation source.

U2 - 10.1107/S0907444902002676

DO - 10.1107/S0907444902002676

M3 - Article

C2 - 11914502

SN - 0907-4449

VL - 58

SP - 704

EP - 705

JO - Acta Crystallographica Section D: Biological Crystallography

JF - Acta Crystallographica Section D: Biological Crystallography

IS - 4

ER -

Crystallization and preliminary crystallographic analysis of a novel cytochrome P450 from Mycobacterium tuberculosis

Abstract

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