Crystallization and preliminary diffraction studies of pentaerythritol tetranitrate reductase from Enterobacter cloacae PB2

Peter C E Moody, Nita Shikotra, Christopher E. French, Neil C. Bruce, Nigel S. Scrutton

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Pentaerythritol tetranitrate (PETN) reductase of Enterobacter cloacae PB2, a flavoprotein involved in the biodegradation of the explosive PETN, ethylene glycol dinitrate (EGDN) and glycerol trinitrate (GTN), was purified from an overexpressing strain of E. coli and crystallized at 293 K using the sitting-drop vapour-diffusion method. Diffraction data can be seen at 1.8 Å. The primitive orthorhombic cell has a monomer in the asymmetric unit. Preliminary molecular-replacement calculations have been performed using a search model based on Old Yellow enzyme.
    Original languageEnglish
    Pages (from-to)675-677
    Number of pages2
    JournalActa Crystallographica Section D: Biological Crystallography
    Volume54
    Issue number4
    DOIs
    Publication statusPublished - 1 Jul 1998

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