Crystallization and preliminary X-ray analysis of an anti-staphylococcal nuclease-staphylococcal nuclease complex and of a second anti-staphylococcal nuclease antibody

ChiehYing Y. Chang; Patricia Bossart Whitaker; Lydia Tabernero; Howard Einspahr; Lisa Workman; David C. Benjamin; Steven Sheriff

doi:10.1006/jmbi.1994.1358

Crystallization and preliminary X-ray analysis of an anti-staphylococcal nuclease-staphylococcal nuclease complex and of a second anti-staphylococcal nuclease antibody

ChiehYing Y. Chang, Patricia Bossart Whitaker, Lydia Tabernero, Howard Einspahr, Lisa Workman, David C. Benjamin, Steven Sheriff

Research output: Contribution to journal › Article › peer-review

Abstract

The Fab fragments of several monoclonal antibodies that bind Staphylococcal nuclease have been screened for crystallization conditions. Two of these, N10 and N25, have been crystallized in forms suitable for X-ray structural analysis. The anti-Staphylococcal nuclease antibody complex N10 Fab-nuclease crystallizes with symmetry consistent with space group C2 and cell parameters of a = 234.7 Å; b = 43.5 Å; c = 74.4 Å; β = 106.4°. A second anti-Staphylococcal nuclease antibody, N25, although crystallized starting with the Fab-nuclease complex, apparently crystallizes as uncomplexed N25 Fab with symmetry consistent with space group P3121 (or its enantiomorph P3221) and cell parameters of a = b = 80.9 Å; c = 138.4 Å.

Original language	English
Pages (from-to)	154-157
Number of pages	3
Journal	Journal of molecular biology
Volume	239
Issue number	1
DOIs	https://doi.org/10.1006/jmbi.1994.1358
Publication status	Published - 1994

Keywords

antibodies
antibody-antigen complexes
crystallization
Fabs
X-ray crystallography

Access to Document

10.1006/jmbi.1994.1358

Cite this

@article{f0f6ee62fc8b44e98093db5f3c2b4488,

title = "Crystallization and preliminary X-ray analysis of an anti-staphylococcal nuclease-staphylococcal nuclease complex and of a second anti-staphylococcal nuclease antibody",

abstract = "The Fab fragments of several monoclonal antibodies that bind Staphylococcal nuclease have been screened for crystallization conditions. Two of these, N10 and N25, have been crystallized in forms suitable for X-ray structural analysis. The anti-Staphylococcal nuclease antibody complex N10 Fab-nuclease crystallizes with symmetry consistent with space group C2 and cell parameters of a = 234.7 {\AA}; b = 43.5 {\AA}; c = 74.4 {\AA}; β = 106.4°. A second anti-Staphylococcal nuclease antibody, N25, although crystallized starting with the Fab-nuclease complex, apparently crystallizes as uncomplexed N25 Fab with symmetry consistent with space group P3121 (or its enantiomorph P3221) and cell parameters of a = b = 80.9 {\AA}; c = 138.4 {\AA}.",

keywords = "antibodies, antibody-antigen complexes, crystallization, Fabs, X-ray crystallography",

author = "Chang, {ChiehYing Y.} and Whitaker, {Patricia Bossart} and Lydia Tabernero and Howard Einspahr and Lisa Workman and Benjamin, {David C.} and Steven Sheriff",

year = "1994",

doi = "10.1006/jmbi.1994.1358",

language = "English",

volume = "239",

pages = "154--157",

journal = "Journal of molecular biology",

issn = "0022-2836",

publisher = "Academic Press",

number = "1",

}

Crystallization and preliminary X-ray analysis of an anti-staphylococcal nuclease-staphylococcal nuclease complex and of a second anti-staphylococcal nuclease antibody. / Chang, ChiehYing Y.; Whitaker, Patricia Bossart; Tabernero, Lydia et al.
In: Journal of molecular biology, Vol. 239, No. 1, 1994, p. 154-157.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Crystallization and preliminary X-ray analysis of an anti-staphylococcal nuclease-staphylococcal nuclease complex and of a second anti-staphylococcal nuclease antibody

AU - Chang, ChiehYing Y.

AU - Whitaker, Patricia Bossart

AU - Tabernero, Lydia

AU - Einspahr, Howard

AU - Workman, Lisa

AU - Benjamin, David C.

AU - Sheriff, Steven

PY - 1994

Y1 - 1994

N2 - The Fab fragments of several monoclonal antibodies that bind Staphylococcal nuclease have been screened for crystallization conditions. Two of these, N10 and N25, have been crystallized in forms suitable for X-ray structural analysis. The anti-Staphylococcal nuclease antibody complex N10 Fab-nuclease crystallizes with symmetry consistent with space group C2 and cell parameters of a = 234.7 Å; b = 43.5 Å; c = 74.4 Å; β = 106.4°. A second anti-Staphylococcal nuclease antibody, N25, although crystallized starting with the Fab-nuclease complex, apparently crystallizes as uncomplexed N25 Fab with symmetry consistent with space group P3121 (or its enantiomorph P3221) and cell parameters of a = b = 80.9 Å; c = 138.4 Å.

AB - The Fab fragments of several monoclonal antibodies that bind Staphylococcal nuclease have been screened for crystallization conditions. Two of these, N10 and N25, have been crystallized in forms suitable for X-ray structural analysis. The anti-Staphylococcal nuclease antibody complex N10 Fab-nuclease crystallizes with symmetry consistent with space group C2 and cell parameters of a = 234.7 Å; b = 43.5 Å; c = 74.4 Å; β = 106.4°. A second anti-Staphylococcal nuclease antibody, N25, although crystallized starting with the Fab-nuclease complex, apparently crystallizes as uncomplexed N25 Fab with symmetry consistent with space group P3121 (or its enantiomorph P3221) and cell parameters of a = b = 80.9 Å; c = 138.4 Å.

KW - antibodies

KW - antibody-antigen complexes

KW - crystallization

KW - Fabs

KW - X-ray crystallography

U2 - 10.1006/jmbi.1994.1358

DO - 10.1006/jmbi.1994.1358

M3 - Article

C2 - 8196044

SN - 0022-2836

VL - 239

SP - 154

EP - 157

JO - Journal of molecular biology

JF - Journal of molecular biology

IS - 1

ER -

Crystallization and preliminary X-ray analysis of an anti-staphylococcal nuclease-staphylococcal nuclease complex and of a second anti-staphylococcal nuclease antibody

Abstract

Keywords

Access to Document

Fingerprint

Cite this