Crystallization of microsomal triglyceride transfer protein from bovine liver

Shari L. Ohringer; Haris Jamil; Ching Hsuen Chu; Lydia Tabernero; Howard M. Einspahr; John R. Wetterau; John S. Sack

doi:10.1107/S0907444995011486

Crystallization of microsomal triglyceride transfer protein from bovine liver

Shari L. Ohringer, Haris Jamil, Ching Hsuen Chu, Lydia Tabernero, Howard M. Einspahr, John R. Wetterau, John S. Sack

Research output: Contribution to journal › Article › peer-review

Abstract

The microsomal triglyceride transfer protein (MTP) is a heterodimeric lipid transfer protein required for the assembly of plasma very low density lipoproteins in the liver and chylomicrons in the intestine. Bovine MTP was purified by a modification of a previously published procedure and crystals of MTP were grown reproducibly with polyethylene glycol as a precipitant at pH 7.0. MTP crystals, which diffract to Bragg spacings of better than 3.2 A, have the symmetry of space group P212121 with refined lattice constants of a=88.7, b=100.9 and c=201.1 Å, with one heterodimer per asymmetric unit.

Original language	English
Pages (from-to)	224-225
Number of pages	1
Journal	Acta Crystallographica Section D: Biological Crystallography
Volume	52
Issue number	1
DOIs	https://doi.org/10.1107/S0907444995011486
Publication status	Published - 1 Jan 1996

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title = "Crystallization of microsomal triglyceride transfer protein from bovine liver",

abstract = "The microsomal triglyceride transfer protein (MTP) is a heterodimeric lipid transfer protein required for the assembly of plasma very low density lipoproteins in the liver and chylomicrons in the intestine. Bovine MTP was purified by a modification of a previously published procedure and crystals of MTP were grown reproducibly with polyethylene glycol as a precipitant at pH 7.0. MTP crystals, which diffract to Bragg spacings of better than 3.2 A, have the symmetry of space group P212121 with refined lattice constants of a=88.7, b=100.9 and c=201.1 {\AA}, with one heterodimer per asymmetric unit.",

author = "Ohringer, {Shari L.} and Haris Jamil and Chu, {Ching Hsuen} and Lydia Tabernero and Einspahr, {Howard M.} and Wetterau, {John R.} and Sack, {John S.}",

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T1 - Crystallization of microsomal triglyceride transfer protein from bovine liver

AU - Ohringer, Shari L.

AU - Jamil, Haris

AU - Chu, Ching Hsuen

AU - Tabernero, Lydia

AU - Einspahr, Howard M.

AU - Wetterau, John R.

AU - Sack, John S.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - The microsomal triglyceride transfer protein (MTP) is a heterodimeric lipid transfer protein required for the assembly of plasma very low density lipoproteins in the liver and chylomicrons in the intestine. Bovine MTP was purified by a modification of a previously published procedure and crystals of MTP were grown reproducibly with polyethylene glycol as a precipitant at pH 7.0. MTP crystals, which diffract to Bragg spacings of better than 3.2 A, have the symmetry of space group P212121 with refined lattice constants of a=88.7, b=100.9 and c=201.1 Å, with one heterodimer per asymmetric unit.

AB - The microsomal triglyceride transfer protein (MTP) is a heterodimeric lipid transfer protein required for the assembly of plasma very low density lipoproteins in the liver and chylomicrons in the intestine. Bovine MTP was purified by a modification of a previously published procedure and crystals of MTP were grown reproducibly with polyethylene glycol as a precipitant at pH 7.0. MTP crystals, which diffract to Bragg spacings of better than 3.2 A, have the symmetry of space group P212121 with refined lattice constants of a=88.7, b=100.9 and c=201.1 Å, with one heterodimer per asymmetric unit.

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DO - 10.1107/S0907444995011486

M3 - Article

SN - 0907-4449

VL - 52

SP - 224

EP - 225

JO - Acta Crystallographica Section D: Biological Crystallography

JF - Acta Crystallographica Section D: Biological Crystallography

IS - 1

ER -

Crystallization of microsomal triglyceride transfer protein from bovine liver

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