Crystallization of microsomal triglyceride transfer protein from bovine liver

Shari L. Ohringer; Haris Jamil; Ching Hsuen Chu; Lydia Tabernero; Howard M. Einspahr; John R. Wetterau; John S. Sack

doi:10.1107/S0907444995011486

Crystallization of microsomal triglyceride transfer protein from bovine liver

Shari L. Ohringer
, Haris Jamil
, Ching Hsuen Chu
, Lydia Tabernero
, Howard M. Einspahr
, John R. Wetterau
, John S. Sack

Research output: Contribution to journal › Article › peer-review

Abstract

The microsomal triglyceride transfer protein (MTP) is a heterodimeric lipid transfer protein required for the assembly of plasma very low density lipoproteins in the liver and chylomicrons in the intestine. Bovine MTP was purified by a modification of a previously published procedure and crystals of MTP were grown reproducibly with polyethylene glycol as a precipitant at pH 7.0. MTP crystals, which diffract to Bragg spacings of better than 3.2 A, have the symmetry of space group P212121 with refined lattice constants of a=88.7, b=100.9 and c=201.1 Å, with one heterodimer per asymmetric unit.

Original language	English
Pages (from-to)	224-225
Number of pages	1
Journal	Acta Crystallographica Section D: Biological Crystallography
Volume	52
Issue number	1
DOIs	https://doi.org/10.1107/S0907444995011486
Publication status	Published - 1 Jan 1996

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title = "Crystallization of microsomal triglyceride transfer protein from bovine liver",

abstract = "The microsomal triglyceride transfer protein (MTP) is a heterodimeric lipid transfer protein required for the assembly of plasma very low density lipoproteins in the liver and chylomicrons in the intestine. Bovine MTP was purified by a modification of a previously published procedure and crystals of MTP were grown reproducibly with polyethylene glycol as a precipitant at pH 7.0. MTP crystals, which diffract to Bragg spacings of better than 3.2 A, have the symmetry of space group P212121 with refined lattice constants of a=88.7, b=100.9 and c=201.1 {\AA}, with one heterodimer per asymmetric unit.",

author = "Ohringer, \{Shari L.\} and Haris Jamil and Chu, \{Ching Hsuen\} and Lydia Tabernero and Einspahr, \{Howard M.\} and Wetterau, \{John R.\} and Sack, \{John S.\}",

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T1 - Crystallization of microsomal triglyceride transfer protein from bovine liver

AU - Ohringer, Shari L.

AU - Jamil, Haris

AU - Chu, Ching Hsuen

AU - Tabernero, Lydia

AU - Einspahr, Howard M.

AU - Wetterau, John R.

AU - Sack, John S.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - The microsomal triglyceride transfer protein (MTP) is a heterodimeric lipid transfer protein required for the assembly of plasma very low density lipoproteins in the liver and chylomicrons in the intestine. Bovine MTP was purified by a modification of a previously published procedure and crystals of MTP were grown reproducibly with polyethylene glycol as a precipitant at pH 7.0. MTP crystals, which diffract to Bragg spacings of better than 3.2 A, have the symmetry of space group P212121 with refined lattice constants of a=88.7, b=100.9 and c=201.1 Å, with one heterodimer per asymmetric unit.

AB - The microsomal triglyceride transfer protein (MTP) is a heterodimeric lipid transfer protein required for the assembly of plasma very low density lipoproteins in the liver and chylomicrons in the intestine. Bovine MTP was purified by a modification of a previously published procedure and crystals of MTP were grown reproducibly with polyethylene glycol as a precipitant at pH 7.0. MTP crystals, which diffract to Bragg spacings of better than 3.2 A, have the symmetry of space group P212121 with refined lattice constants of a=88.7, b=100.9 and c=201.1 Å, with one heterodimer per asymmetric unit.

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DO - 10.1107/S0907444995011486

M3 - Article

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VL - 52

SP - 224

EP - 225

JO - Acta Crystallographica Section D: Biological Crystallography

JF - Acta Crystallographica Section D: Biological Crystallography

IS - 1

ER -

Crystallization of microsomal triglyceride transfer protein from bovine liver

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