CSF pretreatment and the diagnosis of herpes encephalitis using the polymerase chain reaction

C Dennett; P E Klapper; G M Cleator; A G Lewis

doi:10.1016/0166-0934(91)90125-j

CSF pretreatment and the diagnosis of herpes encephalitis using the polymerase chain reaction

C Dennett, P E Klapper, G M Cleator, A G Lewis

University of Manchester

Research output: Contribution to journal › Article › peer-review

Abstract

A number of techniques for extraction of DNA prior to polymerase chain reaction (PCR) amplification of herpes simplex virus (HSV) DNA in cerebrospinal fluid (CSF) were compared to the use of "native" CSF in the PCR reaction. The results indicate that extraction of DNA (which allows efficient removal of inhibitors of Taq polymerase) is an essential pre-requisite of the PCR detection of CSF HSV DNA.

Original language	English
Pages (from-to)	101-4
Number of pages	4
Journal	Journal of Virological Methods
Volume	34
Issue number	1
DOIs	https://doi.org/10.1016/0166-0934(91)90125-j
Publication status	Published - 1 Sept 1991

Keywords

DNA, Viral/cerebrospinal fluid
Encephalitis/diagnosis
Herpes Simplex/cerebrospinal fluid
Humans
Polymerase Chain Reaction/methods
Simplexvirus/genetics

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10.1016/0166-0934(91)90125-j

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abstract = "A number of techniques for extraction of DNA prior to polymerase chain reaction (PCR) amplification of herpes simplex virus (HSV) DNA in cerebrospinal fluid (CSF) were compared to the use of {"}native{"} CSF in the PCR reaction. The results indicate that extraction of DNA (which allows efficient removal of inhibitors of Taq polymerase) is an essential pre-requisite of the PCR detection of CSF HSV DNA.",

keywords = "DNA, Viral/cerebrospinal fluid, Encephalitis/diagnosis, Herpes Simplex/cerebrospinal fluid, Humans, Polymerase Chain Reaction/methods, Simplexvirus/genetics",

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AU - Klapper, P E

AU - Cleator, G M

AU - Lewis, A G

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Y1 - 1991/9/1

N2 - A number of techniques for extraction of DNA prior to polymerase chain reaction (PCR) amplification of herpes simplex virus (HSV) DNA in cerebrospinal fluid (CSF) were compared to the use of "native" CSF in the PCR reaction. The results indicate that extraction of DNA (which allows efficient removal of inhibitors of Taq polymerase) is an essential pre-requisite of the PCR detection of CSF HSV DNA.

AB - A number of techniques for extraction of DNA prior to polymerase chain reaction (PCR) amplification of herpes simplex virus (HSV) DNA in cerebrospinal fluid (CSF) were compared to the use of "native" CSF in the PCR reaction. The results indicate that extraction of DNA (which allows efficient removal of inhibitors of Taq polymerase) is an essential pre-requisite of the PCR detection of CSF HSV DNA.

KW - DNA, Viral/cerebrospinal fluid

KW - Encephalitis/diagnosis

KW - Herpes Simplex/cerebrospinal fluid

KW - Humans

KW - Polymerase Chain Reaction/methods

KW - Simplexvirus/genetics

U2 - 10.1016/0166-0934(91)90125-j

DO - 10.1016/0166-0934(91)90125-j

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SP - 101

EP - 104

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 1

ER -

CSF pretreatment and the diagnosis of herpes encephalitis using the polymerase chain reaction

Abstract

Keywords

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