Cultivation of Heligmosomoides polygyrus: an immunomodulatory nematode parasite and its secreted products.

Chris J C Johnston; Elaine Robertson; Yvonne Harcus; John R Grainger; Gillian Coakley; Danielle J Smyth; Henry J McSorley; Rick Maizels

doi:10.3791/52412

Cultivation of Heligmosomoides polygyrus: an immunomodulatory nematode parasite and its secreted products.

Chris J C Johnston, Elaine Robertson, Yvonne Harcus, John R Grainger, Gillian Coakley, Danielle J Smyth, Henry J McSorley, Rick Maizels

Research output: Contribution to journal › Article › peer-review

Abstract

Heligmosomoides polygyrus (formerly known as Nematospiroides dubius, and also referred to by some as H. bakeri) is a gastrointestinal helminth that employs multiple immunomodulatory mechanisms to establish chronic infection in mice and closely resembles prevalent human helminth infections. H. polygyrus has been studied extensively in the field of helminth-derived immune regulation and has been found to potently suppress experimental models of allergy and autoimmunity (both with active infection and isolated secreted products). The protocol described in this paper outlines management of the H. polygyrus life cycle for consistent production of L3 larvae, recovery of adult parasites, and collection of their excretory-secretory products (HES).

Original language	English
Journal	Journal of visualized experiments : JoVE
Issue number	98
DOIs	https://doi.org/10.3791/52412
Publication status	Published - 2015

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title = "Cultivation of Heligmosomoides polygyrus: an immunomodulatory nematode parasite and its secreted products.",

abstract = "Heligmosomoides polygyrus (formerly known as Nematospiroides dubius, and also referred to by some as H. bakeri) is a gastrointestinal helminth that employs multiple immunomodulatory mechanisms to establish chronic infection in mice and closely resembles prevalent human helminth infections. H. polygyrus has been studied extensively in the field of helminth-derived immune regulation and has been found to potently suppress experimental models of allergy and autoimmunity (both with active infection and isolated secreted products). The protocol described in this paper outlines management of the H. polygyrus life cycle for consistent production of L3 larvae, recovery of adult parasites, and collection of their excretory-secretory products (HES).",

author = "Johnston, {Chris J C} and Elaine Robertson and Yvonne Harcus and Grainger, {John R} and Gillian Coakley and Smyth, {Danielle J} and McSorley, {Henry J} and Rick Maizels",

note = ", Biotechnology and Biological Sciences Research Council, United Kingdom, Wellcome Trust, United Kingdom",

year = "2015",

doi = "10.3791/52412",

language = "English",

journal = "Journal of visualized experiments : JoVE",

publisher = "MyJoVE Corporation",

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T1 - Cultivation of Heligmosomoides polygyrus: an immunomodulatory nematode parasite and its secreted products.

AU - Johnston, Chris J C

AU - Robertson, Elaine

AU - Harcus, Yvonne

AU - Grainger, John R

AU - Coakley, Gillian

AU - Smyth, Danielle J

AU - McSorley, Henry J

AU - Maizels, Rick

N1 - , Biotechnology and Biological Sciences Research Council, United Kingdom, Wellcome Trust, United Kingdom

PY - 2015

Y1 - 2015

N2 - Heligmosomoides polygyrus (formerly known as Nematospiroides dubius, and also referred to by some as H. bakeri) is a gastrointestinal helminth that employs multiple immunomodulatory mechanisms to establish chronic infection in mice and closely resembles prevalent human helminth infections. H. polygyrus has been studied extensively in the field of helminth-derived immune regulation and has been found to potently suppress experimental models of allergy and autoimmunity (both with active infection and isolated secreted products). The protocol described in this paper outlines management of the H. polygyrus life cycle for consistent production of L3 larvae, recovery of adult parasites, and collection of their excretory-secretory products (HES).

AB - Heligmosomoides polygyrus (formerly known as Nematospiroides dubius, and also referred to by some as H. bakeri) is a gastrointestinal helminth that employs multiple immunomodulatory mechanisms to establish chronic infection in mice and closely resembles prevalent human helminth infections. H. polygyrus has been studied extensively in the field of helminth-derived immune regulation and has been found to potently suppress experimental models of allergy and autoimmunity (both with active infection and isolated secreted products). The protocol described in this paper outlines management of the H. polygyrus life cycle for consistent production of L3 larvae, recovery of adult parasites, and collection of their excretory-secretory products (HES).

U2 - 10.3791/52412

DO - 10.3791/52412

M3 - Article

C2 - 25867600

JO - Journal of visualized experiments : JoVE

JF - Journal of visualized experiments : JoVE

IS - 98

ER -

Cultivation of Heligmosomoides polygyrus: an immunomodulatory nematode parasite and its secreted products.

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