Culture of Human Monocyte-Derived Macrophages

Aoife Kelly, Aleksander M Grabiec, Mark Travis

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


The study of human macrophages is often hampered by access to tissue and inability of this cell type to survive in vitro following isolation. The culture of human monocyte-derived macrophages (MDMs) represents a tool to study macrophages, with monocytes known to give rise to tissue macrophages influenced by certain environmental cues. Here we describe a method of culturing monocyte-derived macrophages from CD14+ blood monocytes and polarization toward different macrophage phenotypes.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Number of pages11
Publication statusPublished - 2018

Publication series

NameMethods in molecular biology (Clifton, N.J.)
PublisherHumana Press, Inc.
ISSN (Print)1064-3745

Research Beacons, Institutes and Platforms

  • Lydia Becker Institute


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