Culture of Human Monocyte-Derived Macrophages

Aoife Kelly, Aleksander M Grabiec, Mark Travis

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

The study of human macrophages is often hampered by access to tissue and inability of this cell type to survive in vitro following isolation. The culture of human monocyte-derived macrophages (MDMs) represents a tool to study macrophages, with monocytes known to give rise to tissue macrophages influenced by certain environmental cues. Here we describe a method of culturing monocyte-derived macrophages from CD14+ blood monocytes and polarization toward different macrophage phenotypes.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages1-11
Number of pages11
Volume1784
DOIs
Publication statusPublished - 2018

Publication series

NameMethods in molecular biology (Clifton, N.J.)
PublisherHumana Press, Inc.
ISSN (Print)1064-3745

Research Beacons, Institutes and Platforms

  • Lydia Becker Institute

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