Curcumin downregulates aquaporin-1 expression in cultured rat choroid plexus cells

Mohammad Nabiuni, Zahra Nazari, Zahra Safaeinejad, Bahram Delfan, Jaleel A. Miyan

    Research output: Contribution to journalArticlepeer-review


    Aquaporin-1 (AQP1) is a water channel that is highly expressed on the apical side of the choroid plexus epithelium (CP) and thought to be one of the major pathways for the high water permeability of this structure. Blockade of AQP1 in the CP reduce the production of cerebrospinal fluid (CSF). Downregulation of AQP1 might be protective against some neurological disorders correlated with increased intracranial pressure and/or poor drainage of CSF. Curcumin, the major constituent of the rhizome of Curcuma longa, has been shown to inhibit potassium channels, Na+-K+ ATPase, as well as AQP3 in some cells. We therefore speculated that curcumin might be a useful tool to inhibit and/or decrease AQP1, and thus might be useful in the regulation of CSF production in pathophysiological conditions, including traumatic brain injury, hydrocephalus, stroke, systemic hyponatremia, acute cerebral edema, and hypertension. Choroidal epithelial cells of the lateral ventricle of Wistar rats were isolated and grown in in-vitro cultures for 24 h. Curcumin was then added to the medium at different concentrations, and the cell viability tested by the (3,4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide assay. Additional wells of cells were tested for AQP1 protein expression using immunocytochemistry, immunoblotting, and flow cytometry. Our results showed that curcumin treatment decreases AQP1 expression in rat choroid epithelium cells in a dose-dependent manner. We conclude that curcumin may be a useful tool to regulate CSF production in pathophysiological conditions such as hydrocephalus, systemic hyponatremia, hypertension, and other neurological conditions. © Mary Ann Liebert, Inc. and Korean Society of Food Science and Nutrition.
    Original languageEnglish
    Pages (from-to)504-510
    Number of pages6
    JournalJournal of Medicinal Food
    Issue number6
    Publication statusPublished - 1 Jun 2013


    • AQP1
    • Cerebrospinal fluid
    • Choroid plexus
    • Curcumin


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