Current perspectives and recommendations for the development of mass spectrometry methods for the determination of allergens in foods

Phil E. Johnson, Sabine Baumgartner, Thomas Aldick, Conrad Bessant, Valeria Giosafatto, Julia Heick, Gianfranco Mamone, Gavin O'Connor, Roland Poms, Bert Popping, Andreas Reuter, Franz Ulberth, Andrew Watson, Linda Monaci, E. N Clare Mills

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Allergen detection and quantification is an essential part of allergen management as practiced by food manufacturers. Recently, protein MS methods (in particular, multiple reaction monitoring experiments) have begun to be adopted by the allergen detection community to provide an alternative technique to ELISA and PCR methods. MS analysis of proteins in foods provides additional challenges to the analyst, both in terms of experimental design and methodology: (1) choice of analyte, including multiplexing to simultaneously detect several biologically relevant molecules able to trigger allergic reactions; (2) choice of processing stable peptide markers for different target analytes that should be placed in publicly available databases; (3) markers allowing quantification (e.g., through standard addition or isotopically labeled peptide standards); (4) optimization of protease digestion protocols to ensure reproducible and robust method development; and (5) effective validation of methods and harmonization of results through the use of naturally incurred reference materials spanning several types of food matrix. © 2012 Publishing Technology.
    Original languageEnglish
    Pages (from-to)1026-1033
    Number of pages7
    JournalJournal of AOAC INTERNATIONAL
    Volume94
    Issue number4
    Publication statusPublished - Jul 2011

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