Cyclic AMP inhibits macrophage suppressor function and enhances lymphocyte proliferation

W. M. Gonsalkorale, M. J. Dascombe, I. V. Hutchinson

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The effects of increasing the level of cyclic AMP (cAMP) activity on lymphocyte proliferation in the rat mixed lymphocyte reaction (MLR) were investigated. Dibutyryl cAMP (dbcAMP) and prostaglandin E2 (PGE2) produced a dose-dependent reduction in proliferation in the lymph node (LN) MLR, but produced a substantial increase in proliferation in the spleen MLR at the lower concentrations used (10-5-10-4 M dbcAMP; 10-7-10-6 M PGE2). Enhancement of proliferation was dependent on the presence of macrophages and was probably due to inhibition of macrophage activation. This was based on the following findings: (1) spleen MLR proliferation was lower than that in the LN MLR; (2) depletion of spleen macrophages proliferation in the spleen MLR and addition of these macrophages to the LN MLR reduced proliferation; (3) macrophage depletion from the spleen MLR abolished the proliferation-enhancing effect of dbcAMP. In conclusion, cAMP enhances lymphocyte proliferation in this system, apparently as a consequence of suppressing the inhibitory influence of macrophages.
    Original languageEnglish
    Pages (from-to)611-616
    Number of pages5
    JournalImmunology
    Volume80
    Issue number4
    Publication statusPublished - 1993

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