Cyclin D1 integrates estrogen-mediated DNA damage repair signaling.

Zhiping Li, Ke Chen, Xuanmao Jiao, Chenguang Wang, Nicole E Willmarth, Mathew C Casimiro, Weihua Li, Xiaoming Ju, Sung Hoon Kim, Michael P Lisanti, John A Katzenellenbogen, Richard G Pestell

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The cyclin D1 gene encodes the regulatory subunit of a holoenyzme that phosphorylates the retinoblastoma protein (pRb) and nuclear respiratory factor (NRF1) proteins. The abundance of cyclin D1 determines estrogen-dependent gene expression in the mammary gland of mice. Using estradiol (E2) and an E2-dendrimer conjugate that is excluded from the nucleus, we demonstrate that E2 delays the DNA damage response (DDR) via an extranuclear mechanism. The E2-induced DDR required extranuclear cyclin D1, which bound ERα at the cytoplasmic membrane and augmented AKT phosphorylation (Ser473) and γH2AX foci formation. In the nucleus, E2 inhibited, whereas cyclin D1 enhanced homology-directed DNA repair. Cyclin D1 was recruited to γH2AX foci by E2 and induced Rad51 expression. Cyclin D1 governs an essential role in the E2-dependent DNA damage response via a novel extranuclear function. The dissociable cytoplasmic function to delay the E2-mediated DDR together with the nuclear enhancement of DNA repair uncovers a novel extranuclear function of cyclin D1 that may contribute to the role of E2 in breast tumorigenesis.
    Original languageEnglish
    JournalCancer Research
    Volume74
    Issue number14
    DOIs
    Publication statusPublished - 15 Jul 2014

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