Cyclin e is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells

Jennifer Munkley; Nikki A. Copeland; Victoria Moignard; John R.P. Knight; Erin Greaves; Simon A. Ramsbottom; Mary E. Pownall; Jennifer Southgate; Justin F.X. Ainscough; Dawn Coverley

doi:10.1093/nar/gkq1190

Cyclin e is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells

Jennifer Munkley, Nikki A. Copeland, Victoria Moignard, John R.P. Knight, Erin Greaves, Simon A. Ramsbottom, Mary E. Pownall, Jennifer Southgate, Justin F.X. Ainscough, Dawn Coverley

Division of Cancer Sciences (L5)

Research output: Contribution to journal › Article › peer-review

Abstract

Cyclin E supports pre-replication complex (pre-RC) assembly, while cyclin A-associated kinase activates DNA synthesis. We show that cyclin E, but not A, is mounted upon the nuclear matrix in sub-nuclear foci in differentiated vertebrate cells, but not in undifferentiated cells or cancer cells. In murine embryonic stem cells, Xenopus embryos and human urothelial cells, cyclin E is recruited to the nuclear matrix as cells differentiate and this can be manipulated in vitro. This suggests that pre-RC assembly becomes spatially restricted as template usage is defined. Furthermore, failure to become restricted may contribute to the plasticity of cancer cells.

Original language	English
Pages (from-to)	2671-2677
Number of pages	7
Journal	Nucleic acids research
Volume	39
Issue number	7
DOIs	https://doi.org/10.1093/nar/gkq1190
Publication status	Published - Apr 2011

Research Beacons, Institutes and Platforms

Manchester Cancer Research Centre

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1093/nar/gkq1190

Cite this

@article{29d7f9f1949e4eca85a8bdd9f8b840f7,

title = "Cyclin e is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells",

abstract = "Cyclin E supports pre-replication complex (pre-RC) assembly, while cyclin A-associated kinase activates DNA synthesis. We show that cyclin E, but not A, is mounted upon the nuclear matrix in sub-nuclear foci in differentiated vertebrate cells, but not in undifferentiated cells or cancer cells. In murine embryonic stem cells, Xenopus embryos and human urothelial cells, cyclin E is recruited to the nuclear matrix as cells differentiate and this can be manipulated in vitro. This suggests that pre-RC assembly becomes spatially restricted as template usage is defined. Furthermore, failure to become restricted may contribute to the plasticity of cancer cells.",

author = "Jennifer Munkley and Copeland, {Nikki A.} and Victoria Moignard and Knight, {John R.P.} and Erin Greaves and Ramsbottom, {Simon A.} and Pownall, {Mary E.} and Jennifer Southgate and Ainscough, {Justin F.X.} and Dawn Coverley",

note = "Funding Information: Biotechnology and Biosciences Research Council (BBSRC); Yorkshire Cancer Research; York Against Cancer (to J.S.); British Heart Foundation (to J.F.-X.A.). Funding for the open access charge: BBSRC; Yorkshire Cancer Research, partial.",

year = "2011",

month = apr,

doi = "10.1093/nar/gkq1190",

language = "English",

volume = "39",

pages = "2671--2677",

journal = "Nucleic acids research",

issn = "0305-1048",

publisher = "Oxford University Press",

number = "7",

}

TY - JOUR

T1 - Cyclin e is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells

AU - Munkley, Jennifer

AU - Copeland, Nikki A.

AU - Moignard, Victoria

AU - Knight, John R.P.

AU - Greaves, Erin

AU - Ramsbottom, Simon A.

AU - Pownall, Mary E.

AU - Southgate, Jennifer

AU - Ainscough, Justin F.X.

AU - Coverley, Dawn

N1 - Funding Information: Biotechnology and Biosciences Research Council (BBSRC); Yorkshire Cancer Research; York Against Cancer (to J.S.); British Heart Foundation (to J.F.-X.A.). Funding for the open access charge: BBSRC; Yorkshire Cancer Research, partial.

PY - 2011/4

Y1 - 2011/4

N2 - Cyclin E supports pre-replication complex (pre-RC) assembly, while cyclin A-associated kinase activates DNA synthesis. We show that cyclin E, but not A, is mounted upon the nuclear matrix in sub-nuclear foci in differentiated vertebrate cells, but not in undifferentiated cells or cancer cells. In murine embryonic stem cells, Xenopus embryos and human urothelial cells, cyclin E is recruited to the nuclear matrix as cells differentiate and this can be manipulated in vitro. This suggests that pre-RC assembly becomes spatially restricted as template usage is defined. Furthermore, failure to become restricted may contribute to the plasticity of cancer cells.

AB - Cyclin E supports pre-replication complex (pre-RC) assembly, while cyclin A-associated kinase activates DNA synthesis. We show that cyclin E, but not A, is mounted upon the nuclear matrix in sub-nuclear foci in differentiated vertebrate cells, but not in undifferentiated cells or cancer cells. In murine embryonic stem cells, Xenopus embryos and human urothelial cells, cyclin E is recruited to the nuclear matrix as cells differentiate and this can be manipulated in vitro. This suggests that pre-RC assembly becomes spatially restricted as template usage is defined. Furthermore, failure to become restricted may contribute to the plasticity of cancer cells.

UR - http://www.scopus.com/inward/record.url?scp=79954614534&partnerID=8YFLogxK

U2 - 10.1093/nar/gkq1190

DO - 10.1093/nar/gkq1190

M3 - Article

C2 - 21109536

AN - SCOPUS:79954614534

SN - 0305-1048

VL - 39

SP - 2671

EP - 2677

JO - Nucleic acids research

JF - Nucleic acids research

IS - 7

ER -

Cyclin e is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells

Abstract

Research Beacons, Institutes and Platforms

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this