Cytokine fingerprinting of chemical allergens: Species comparisons and statistical analyses

R. J. Dearman, E. V. Warbrick, R. Skinner, I. Kimber

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The cellular and molecular mechanisms that result in the induction of chemical respiratory sensitization are unclear, although there is evidence for the development of T helper (Th) 2 type responses and, in some cases, the production of IgE. We have compared cytokine secretion patterns stimulated by topical exposure of BALB/c strain mice or Brown Norway (BN) strain rats to the reference respiratory allergen trimellitic anhydride (TMA), or to the reference contact allergen 2,4-dinitrochlorobenzene (DNCB). Under conditions where TMA and DNCB provoke similar levels of immune activation [increases in lymph node cell (LNC) cellularity and proliferation] divergent cytokine expression patterns are elicited. TMA-activated LNC isolated from BALB/c mice or BN rats elaborated high levels of the Th2-type cytokines interleukin (IL)-10 and IL-13, but relatively little of the Th1-type cytokines IL-12 or interferon γ. For LNC derived from both species there was a requirement for restimulation in vitro with the mitogen concanavalin A for IL-4 production. Generally, DNCB-stimulated LNC displayed the converse type 1 cytokine phenotype. The cytokine secretion profiles of LNC isolated from BN rats were considerably more variable than those observed for LNC from BALB/c mice. Statistically significant differences (P
    Original languageEnglish
    Pages (from-to)1881-1892
    Number of pages11
    JournalFood and Chemical Toxicology
    Volume40
    Issue number12
    DOIs
    Publication statusPublished - 1 Dec 2002

    Keywords

    • Contact allergy
    • Cytokines
    • IgE
    • Respiratory allergy
    • T cell subsets

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