De Novo Design of Ln (III) Coiled Coils for Imaging Applications

Matthew  Berwick; David Lewis; Andrew Jones; Rosemary Parslow; Timothy R Dafforn; Helen J. Cooper; John Wilkie; Zoe Pikramenou; Melanie Britton; Anna F A Peacock

doi:10.1021/ja408741h

De Novo Design of Ln (III) Coiled Coils for Imaging Applications

Matthew Berwick, David Lewis, Andrew Jones, Rosemary Parslow, Timothy R Dafforn, Helen J. Cooper, John Wilkie, Zoe Pikramenou, Melanie Britton, Anna F A Peacock

Research output: Contribution to journal › Article › peer-review

Abstract

A new peptide sequence (MB1) has been designed which, in the presence of a trivalent lanthanide ion, has been programmed to self-assemble to form a three stranded metallo-coiled coil, Ln(III)(MB1)3. The binding site has been incorporated into the hydrophobic core using natural amino acids, restricting water access to the lanthanide. The resulting terbium coiled coil displays luminescent properties consistent with a lack of first coordination sphere water molecules. Despite this the gadolinium coiled coil, the first to be reported, displays promising magnetic resonance contrast capabilities.

Original language	English
Pages (from-to)	1166-1169
Journal	Journal of the American Chemical Society
Volume	136
Issue number	4
DOIs	https://doi.org/10.1021/ja408741h
Publication status	Published - 2014

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title = "De Novo Design of Ln (III) Coiled Coils for Imaging Applications",

abstract = "A new peptide sequence (MB1) has been designed which, in the presence of a trivalent lanthanide ion, has been programmed to self-assemble to form a three stranded metallo-coiled coil, Ln(III)(MB1)3. The binding site has been incorporated into the hydrophobic core using natural amino acids, restricting water access to the lanthanide. The resulting terbium coiled coil displays luminescent properties consistent with a lack of first coordination sphere water molecules. Despite this the gadolinium coiled coil, the first to be reported, displays promising magnetic resonance contrast capabilities.",

author = "Matthew Berwick and David Lewis and Andrew Jones and Rosemary Parslow and Dafforn, {Timothy R} and Cooper, {Helen J.} and John Wilkie and Zoe Pikramenou and Melanie Britton and Peacock, {Anna F A}",

year = "2014",

doi = "10.1021/ja408741h",

language = "English",

volume = "136",

pages = "1166--1169",

journal = "Journal of the American Chemical Society",

issn = "0002-7863",

publisher = "American Chemical Society",

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TY - JOUR

T1 - De Novo Design of Ln (III) Coiled Coils for Imaging Applications

AU - Berwick, Matthew

AU - Lewis, David

AU - Jones, Andrew

AU - Parslow, Rosemary

AU - Dafforn, Timothy R

AU - Cooper, Helen J.

AU - Wilkie, John

AU - Pikramenou, Zoe

AU - Britton, Melanie

AU - Peacock, Anna F A

PY - 2014

Y1 - 2014

N2 - A new peptide sequence (MB1) has been designed which, in the presence of a trivalent lanthanide ion, has been programmed to self-assemble to form a three stranded metallo-coiled coil, Ln(III)(MB1)3. The binding site has been incorporated into the hydrophobic core using natural amino acids, restricting water access to the lanthanide. The resulting terbium coiled coil displays luminescent properties consistent with a lack of first coordination sphere water molecules. Despite this the gadolinium coiled coil, the first to be reported, displays promising magnetic resonance contrast capabilities.

AB - A new peptide sequence (MB1) has been designed which, in the presence of a trivalent lanthanide ion, has been programmed to self-assemble to form a three stranded metallo-coiled coil, Ln(III)(MB1)3. The binding site has been incorporated into the hydrophobic core using natural amino acids, restricting water access to the lanthanide. The resulting terbium coiled coil displays luminescent properties consistent with a lack of first coordination sphere water molecules. Despite this the gadolinium coiled coil, the first to be reported, displays promising magnetic resonance contrast capabilities.

U2 - 10.1021/ja408741h

DO - 10.1021/ja408741h

M3 - Article

SN - 0002-7863

VL - 136

SP - 1166

EP - 1169

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 4

ER -

De Novo Design of Ln (III) Coiled Coils for Imaging Applications

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