Decreased expression of inhibitory SMAD6 and SMAD7 in keloid scarring

Haiyan Yu; Oliver Bock; Ardeshir Bayat; Mark W. Ferguson; Ulrich Mrowietz

doi:10.1016/j.bjps.2005.06.010

Decreased expression of inhibitory SMAD6 and SMAD7 in keloid scarring

Haiyan Yu, Oliver Bock, Ardeshir Bayat, Mark W. Ferguson, Ulrich Mrowietz

Research output: Contribution to journal › Article › peer-review

Abstract

Keloids are benign skin tumours occurring during wound healing in genetically predisposed patients. They are characterised by an abnormal deposition of extracellular matrix components, in particular collagen. There is evidence that transforming growth factor-beta (TGFβ) is involved in keloid formation. SMAD proteins play a crucial role in TGFβ signaling and in terminating the TGFβ signal by a negative feedback loop through SMAD6 and 7. It is unclear how TGFβ signalling is connected to the pathogenesis of keloids. Therefore, we investigated the expression of SMAD mRNA and proteins in keloids, in normal skin and in normal scars. Dermal fibroblasts were obtained from punch-biopsies of keloids, normal scars and normal skin. Cells were stimulated with TGFβ1 and the expression of SMAD2, 3, 4, 6 and 7 mRNA was analysed by real time RT-PCR. Protein expression was determined by Western blot analysis. Our data demonstrate a decreased mRNA expression of the inhibitory SMAD6 and 7 in keloid fibroblasts as compared to normal scar (p

Original language	English
Pages (from-to)	221-229
Number of pages	8
Journal	Journal of Plastic, Reconstructive and Aesthetic Surgery
Volume	59
Issue number	3
DOIs	https://doi.org/10.1016/j.bjps.2005.06.010
Publication status	Published - Mar 2006

Keywords

Keloids
SMAD
TGFβ
TGFβ signalling

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bjps.2005.06.010

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title = "Decreased expression of inhibitory SMAD6 and SMAD7 in keloid scarring",

abstract = "Keloids are benign skin tumours occurring during wound healing in genetically predisposed patients. They are characterised by an abnormal deposition of extracellular matrix components, in particular collagen. There is evidence that transforming growth factor-beta (TGFβ) is involved in keloid formation. SMAD proteins play a crucial role in TGFβ signaling and in terminating the TGFβ signal by a negative feedback loop through SMAD6 and 7. It is unclear how TGFβ signalling is connected to the pathogenesis of keloids. Therefore, we investigated the expression of SMAD mRNA and proteins in keloids, in normal skin and in normal scars. Dermal fibroblasts were obtained from punch-biopsies of keloids, normal scars and normal skin. Cells were stimulated with TGFβ1 and the expression of SMAD2, 3, 4, 6 and 7 mRNA was analysed by real time RT-PCR. Protein expression was determined by Western blot analysis. Our data demonstrate a decreased mRNA expression of the inhibitory SMAD6 and 7 in keloid fibroblasts as compared to normal scar (p",

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author = "Haiyan Yu and Oliver Bock and Ardeshir Bayat and Ferguson, {Mark W.} and Ulrich Mrowietz",

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doi = "10.1016/j.bjps.2005.06.010",

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TY - JOUR

T1 - Decreased expression of inhibitory SMAD6 and SMAD7 in keloid scarring

AU - Yu, Haiyan

AU - Bock, Oliver

AU - Bayat, Ardeshir

AU - Ferguson, Mark W.

AU - Mrowietz, Ulrich

PY - 2006/3

Y1 - 2006/3

N2 - Keloids are benign skin tumours occurring during wound healing in genetically predisposed patients. They are characterised by an abnormal deposition of extracellular matrix components, in particular collagen. There is evidence that transforming growth factor-beta (TGFβ) is involved in keloid formation. SMAD proteins play a crucial role in TGFβ signaling and in terminating the TGFβ signal by a negative feedback loop through SMAD6 and 7. It is unclear how TGFβ signalling is connected to the pathogenesis of keloids. Therefore, we investigated the expression of SMAD mRNA and proteins in keloids, in normal skin and in normal scars. Dermal fibroblasts were obtained from punch-biopsies of keloids, normal scars and normal skin. Cells were stimulated with TGFβ1 and the expression of SMAD2, 3, 4, 6 and 7 mRNA was analysed by real time RT-PCR. Protein expression was determined by Western blot analysis. Our data demonstrate a decreased mRNA expression of the inhibitory SMAD6 and 7 in keloid fibroblasts as compared to normal scar (p

AB - Keloids are benign skin tumours occurring during wound healing in genetically predisposed patients. They are characterised by an abnormal deposition of extracellular matrix components, in particular collagen. There is evidence that transforming growth factor-beta (TGFβ) is involved in keloid formation. SMAD proteins play a crucial role in TGFβ signaling and in terminating the TGFβ signal by a negative feedback loop through SMAD6 and 7. It is unclear how TGFβ signalling is connected to the pathogenesis of keloids. Therefore, we investigated the expression of SMAD mRNA and proteins in keloids, in normal skin and in normal scars. Dermal fibroblasts were obtained from punch-biopsies of keloids, normal scars and normal skin. Cells were stimulated with TGFβ1 and the expression of SMAD2, 3, 4, 6 and 7 mRNA was analysed by real time RT-PCR. Protein expression was determined by Western blot analysis. Our data demonstrate a decreased mRNA expression of the inhibitory SMAD6 and 7 in keloid fibroblasts as compared to normal scar (p

KW - Keloids

KW - SMAD

KW - TGFβ

KW - TGFβ signalling

U2 - 10.1016/j.bjps.2005.06.010

DO - 10.1016/j.bjps.2005.06.010

M3 - Article

SN - 1748-6815

VL - 59

SP - 221

EP - 229

JO - Journal of Plastic, Reconstructive and Aesthetic Surgery

JF - Journal of Plastic, Reconstructive and Aesthetic Surgery

IS - 3

ER -

Decreased expression of inhibitory SMAD6 and SMAD7 in keloid scarring

Abstract

Keywords

UN SDGs

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