Deep learning for automatic cell detection in wide-field microscopy zebrafish images

B Dong, L Shao, M Da Costa, O Bandmann, Alejandro F Frangi

Research output: Chapter in Book/Conference proceedingConference contributionpeer-review

Abstract

The zebrafish has become a popular experimental model organism for biomedical research. In this paper, a unique framework is proposed for automatically detecting Tyrosine Hydroxylase-containing (TH-labeled) cells in larval zebrafish brain z-stack images recorded through the wide-field microscope. In this framework, a supervised max-pooling Convolutional Neural Network (CNN) is trained to detect cell pixels in regions that are preselected by a Support Vector Machine (SVM) classifier. The results show that the proposed deep-learned method outperforms hand-crafted techniques and demonstrate its potential for automatic cell detection in wide-field microscopy z-stack zebrafish images.

Original languageEnglish
Title of host publication2015 IEEE 12th International Symposium on Biomedical Imaging, ISBI 2015
PublisherIEEE Computer Society
Pages772-776
Number of pages5
ISBN (Electronic)9781479923748
DOIs
Publication statusPublished - 21 Jul 2015
Event12th IEEE International Symposium on Biomedical Imaging, ISBI 2015 - Brooklyn, United States
Duration: 16 Apr 201519 Apr 2015

Publication series

NameProceedings - International Symposium on Biomedical Imaging
Volume2015-July
ISSN (Print)1945-7928
ISSN (Electronic)1945-8452

Conference

Conference12th IEEE International Symposium on Biomedical Imaging, ISBI 2015
Country/TerritoryUnited States
CityBrooklyn
Period16/04/1519/04/15

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