Defining uremic arterial functional abnormalities in patients recently started on haemodialysis: combined in vivo and ex vivo assessment

A M Abushufa; M T Eldehni; A Odudu; P D Evans; S E O'Sullivan; C W McIntyre

doi:10.1371/journal.pone.0113462

Defining uremic arterial functional abnormalities in patients recently started on haemodialysis: combined in vivo and ex vivo assessment

A M Abushufa, M T Eldehni, A Odudu, P D Evans, S E O'Sullivan, C W McIntyre

Research output: Contribution to journal › Article › peer-review

Abstract

Endothelial dysfunction is a key initiating event in vascular disease in chronic kidney disease (CKD) patients and haemodialysis (HD) patients exhibit significant vascular abnormalities. To understand this further, we examined how ex vivo intrinsic function in isolated arteries correlates with in vivo assessments of cardiovascular status in HD patients. Abdominal fat biopsies were obtained from 11 HD patients and 26 non-uremic controls. Subcutaneous arteries were dissected and mounted on a wire myograph, and cumulative concentration-response curves to noradrenalin, endothelin-1, a thromboxane A2 agonist (U46619), angiotensin II, vasopressin, bradykinin (BK), acetylcholine (ACh) and sodium nitroprusside (SNP) were constructed. Pulse wave velocity and blood pressure were measured in HD patients. Enhanced (P600 microm. The potency (pEC50) of U46619 (P600 microm of HD patients. The maximal relaxant response to the endothelium-dependent dilators ACh and BK were lower in HD patients (P

Original language	English
Journal	PLoS ONE
Volume	9
Issue number	12
DOIs	https://doi.org/10.1371/journal.pone.0113462
Publication status	Published - 2014

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title = "Defining uremic arterial functional abnormalities in patients recently started on haemodialysis: combined in vivo and ex vivo assessment",

abstract = "Endothelial dysfunction is a key initiating event in vascular disease in chronic kidney disease (CKD) patients and haemodialysis (HD) patients exhibit significant vascular abnormalities. To understand this further, we examined how ex vivo intrinsic function in isolated arteries correlates with in vivo assessments of cardiovascular status in HD patients. Abdominal fat biopsies were obtained from 11 HD patients and 26 non-uremic controls. Subcutaneous arteries were dissected and mounted on a wire myograph, and cumulative concentration-response curves to noradrenalin, endothelin-1, a thromboxane A2 agonist (U46619), angiotensin II, vasopressin, bradykinin (BK), acetylcholine (ACh) and sodium nitroprusside (SNP) were constructed. Pulse wave velocity and blood pressure were measured in HD patients. Enhanced (P600 microm. The potency (pEC50) of U46619 (P600 microm of HD patients. The maximal relaxant response to the endothelium-dependent dilators ACh and BK were lower in HD patients (P",

author = "Abushufa, {A M} and Eldehni, {M T} and A Odudu and Evans, {P D} and O'Sullivan, {S E} and McIntyre, {C W}",

note = "Abushufa, Adil M Eldehni, Mohamed T Odudu, Aghogho Evans, Philip D O'Sullivan, Saoirse E McIntyre, Chris W eng 2014/12/30 06:00 PLoS One. 2014 Dec 29;9(12):e113462. doi: 10.1371/journal.pone.0113462. eCollection 2014.",

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AU - Abushufa, A M

AU - Eldehni, M T

AU - Odudu, A

AU - Evans, P D

AU - O'Sullivan, S E

AU - McIntyre, C W

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AB - Endothelial dysfunction is a key initiating event in vascular disease in chronic kidney disease (CKD) patients and haemodialysis (HD) patients exhibit significant vascular abnormalities. To understand this further, we examined how ex vivo intrinsic function in isolated arteries correlates with in vivo assessments of cardiovascular status in HD patients. Abdominal fat biopsies were obtained from 11 HD patients and 26 non-uremic controls. Subcutaneous arteries were dissected and mounted on a wire myograph, and cumulative concentration-response curves to noradrenalin, endothelin-1, a thromboxane A2 agonist (U46619), angiotensin II, vasopressin, bradykinin (BK), acetylcholine (ACh) and sodium nitroprusside (SNP) were constructed. Pulse wave velocity and blood pressure were measured in HD patients. Enhanced (P600 microm. The potency (pEC50) of U46619 (P600 microm of HD patients. The maximal relaxant response to the endothelium-dependent dilators ACh and BK were lower in HD patients (P

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Defining uremic arterial functional abnormalities in patients recently started on haemodialysis: combined in vivo and ex vivo assessment

Abstract

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