Degradation of vancomycin in external quality assessment samples is a factor to underestimate its concentration

Yaxin Fan; Yu Wang; Mengting Chen; Yuancheng Chen; Beining Guo; Xiaofen Liu; Hailan Wu; Yi Li; Jiali Hu; Jing Zhang

Degradation of vancomycin in external quality assessment samples is a factor to underestimate its concentration

Yaxin Fan, Yu Wang, Mengting Chen, Yuancheng Chen, Beining Guo, Xiaofen Liu, Hailan Wu, Yi Li, Jiali Hu, Jing Zhang

Huashan Hospital

Research output: Contribution to journal › Article › peer-review

Abstract

Aim: To compare the difference between the measured and target values in vancomycin external quality assessment (EQA) samples and to investigate the factors for underestimating its concentration. Materials & methods: A retrospective analysis of 195 international vancomycin EQA results was performed. Deviations of the concentrations determined by TDx fluorescence polarization immunoassay (FPIA), Axsym FPIA and Architect chemiluminescence microparticle immunoassay (CMIA) method were -2.43, -16.28 and -10.53%, respectively. Chromatographic peaks of the crystalling degradation products appeared in samples with large deviations. Vancomycin were degraded after long-term transporting and high temperature. Conclusion: Vancomycin concentrations measured by Axsym FPIA and Architect CMIA methods were likely to be underestimated. Long-term transporting resulted in low EQA results, suggesting that establishing a local EQA system for vancomycin is essential.

Original language	English
Journal	Bioanalysis
Publication status	Published - 2021
Externally published	Yes

Cite this

@article{95bc4ae4c66b48f3aca6fbdd1b98f5fe,

title = "Degradation of vancomycin in external quality assessment samples is a factor to underestimate its concentration",

abstract = "Aim: To compare the difference between the measured and target values in vancomycin external quality assessment (EQA) samples and to investigate the factors for underestimating its concentration. Materials & methods: A retrospective analysis of 195 international vancomycin EQA results was performed. Deviations of the concentrations determined by TDx fluorescence polarization immunoassay (FPIA), Axsym FPIA and Architect chemiluminescence microparticle immunoassay (CMIA) method were -2.43, -16.28 and -10.53%, respectively. Chromatographic peaks of the crystalling degradation products appeared in samples with large deviations. Vancomycin were degraded after long-term transporting and high temperature. Conclusion: Vancomycin concentrations measured by Axsym FPIA and Architect CMIA methods were likely to be underestimated. Long-term transporting resulted in low EQA results, suggesting that establishing a local EQA system for vancomycin is essential.",

author = "Yaxin Fan and Yu Wang and Mengting Chen and Yuancheng Chen and Beining Guo and Xiaofen Liu and Hailan Wu and Yi Li and Jiali Hu and Jing Zhang",

year = "2021",

language = "English",

journal = "Bioanalysis",

issn = "1757-6180",

publisher = "Taylor & Francis",

}

TY - JOUR

T1 - Degradation of vancomycin in external quality assessment samples is a factor to underestimate its concentration

AU - Fan, Yaxin

AU - Wang, Yu

AU - Chen, Mengting

AU - Chen, Yuancheng

AU - Guo, Beining

AU - Liu, Xiaofen

AU - Wu, Hailan

AU - Li, Yi

AU - Hu, Jiali

AU - Zhang, Jing

PY - 2021

Y1 - 2021

N2 - Aim: To compare the difference between the measured and target values in vancomycin external quality assessment (EQA) samples and to investigate the factors for underestimating its concentration. Materials & methods: A retrospective analysis of 195 international vancomycin EQA results was performed. Deviations of the concentrations determined by TDx fluorescence polarization immunoassay (FPIA), Axsym FPIA and Architect chemiluminescence microparticle immunoassay (CMIA) method were -2.43, -16.28 and -10.53%, respectively. Chromatographic peaks of the crystalling degradation products appeared in samples with large deviations. Vancomycin were degraded after long-term transporting and high temperature. Conclusion: Vancomycin concentrations measured by Axsym FPIA and Architect CMIA methods were likely to be underestimated. Long-term transporting resulted in low EQA results, suggesting that establishing a local EQA system for vancomycin is essential.

AB - Aim: To compare the difference between the measured and target values in vancomycin external quality assessment (EQA) samples and to investigate the factors for underestimating its concentration. Materials & methods: A retrospective analysis of 195 international vancomycin EQA results was performed. Deviations of the concentrations determined by TDx fluorescence polarization immunoassay (FPIA), Axsym FPIA and Architect chemiluminescence microparticle immunoassay (CMIA) method were -2.43, -16.28 and -10.53%, respectively. Chromatographic peaks of the crystalling degradation products appeared in samples with large deviations. Vancomycin were degraded after long-term transporting and high temperature. Conclusion: Vancomycin concentrations measured by Axsym FPIA and Architect CMIA methods were likely to be underestimated. Long-term transporting resulted in low EQA results, suggesting that establishing a local EQA system for vancomycin is essential.

UR - https://pubmed.ncbi.nlm.nih.gov/34789003/

M3 - Article

SN - 1757-6180

JO - Bioanalysis

JF - Bioanalysis

ER -

Degradation of vancomycin in external quality assessment samples is a factor to underestimate its concentration

Abstract

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