Demonstration of catch bonds between an integrin and its ligand

Fang Kong, Andrés J. García, A. Paul Mould, Martin J. Humphries, Cheng Zhu

    Research output: Contribution to journalArticlepeer-review


    Binding of integrins to ligands provides anchorage and signals for the cell, making them prime candidates for mechanosensing molecules. How force regulates integrin-ligand dissociation is unclear. We used atomic force microscopy to measure the force-dependent lifetimes of single bonds between a fibronectin fragment and an integrin α5β1-Fc fusion protein or membrane α5β1. Force prolonged bond lifetimes in the 10-30-pN range, a counterintuitive behavior called catch bonds. Changing cations from Ca2+/Mg2+ to Mg2+/EGTA and to Mn2+ caused longer lifetime in the same 10-30-pN catch bond region. A truncated α5β1 construct containing the headpiece but not the legs formed longer-lived catch bonds that were not affected by cation changes at forces
    Original languageEnglish
    Pages (from-to)1275-1284
    Number of pages9
    JournalJournal of Cell Biology
    Issue number7
    Publication statusPublished - 29 Jun 2009


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