Design of a synthetic yeast genome

Sarah M Richardson; Leslie A Mitchell; Giovanni Stracquadanio; Kun Yang; Jessica S Dymond; James E DiCarlo; Dongwon Lee; Cheng Lai Victor Huang; Srinivasan Chandrasegaran; Yizhi Cai; Jef D Boeke; Joel S Bader

doi:10.1126/science.aaf4557

Design of a synthetic yeast genome

Sarah M Richardson, Leslie A Mitchell, Giovanni Stracquadanio, Kun Yang, Jessica S Dymond, James E DiCarlo, Dongwon Lee, Cheng Lai Victor Huang, Srinivasan Chandrasegaran, Yizhi Cai, Jef D Boeke, Joel S Bader

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Abstract

We describe complete design of a synthetic eukaryotic genome, Sc2.0, a highly modified Saccharomyces cerevisiae genome reduced in size by nearly 8%, with 1.1 megabases of the synthetic genome deleted, inserted, or altered. Sc2.0 chromosome design was implemented with BioStudio, an open-source framework developed for eukaryotic genome design, which coordinates design modifications from nucleotide to genome scales and enforces version control to systematically track edits. To achieve complete Sc2.0 genome synthesis, individual synthetic chromosomes built by Sc2.0 Consortium teams around the world will be consolidated into a single strain by "endoreduplication intercross." Chemically synthesized genomes like Sc2.0 are fully customizable and allow experimentalists to ask otherwise intractable questions about chromosome structure, function, and evolution with a bottom-up design strategy.

Original language	English
Pages (from-to)	1040-1044
Number of pages	5
Journal	Science
Volume	355
Issue number	6329
DOIs	https://doi.org/10.1126/science.aaf4557
Publication status	Published - 10 Mar 2017

Keywords

Journal Article
Research Support, Non-U.S. Gov't

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title = "Design of a synthetic yeast genome",

abstract = "We describe complete design of a synthetic eukaryotic genome, Sc2.0, a highly modified Saccharomyces cerevisiae genome reduced in size by nearly 8%, with 1.1 megabases of the synthetic genome deleted, inserted, or altered. Sc2.0 chromosome design was implemented with BioStudio, an open-source framework developed for eukaryotic genome design, which coordinates design modifications from nucleotide to genome scales and enforces version control to systematically track edits. To achieve complete Sc2.0 genome synthesis, individual synthetic chromosomes built by Sc2.0 Consortium teams around the world will be consolidated into a single strain by {"}endoreduplication intercross.{"} Chemically synthesized genomes like Sc2.0 are fully customizable and allow experimentalists to ask otherwise intractable questions about chromosome structure, function, and evolution with a bottom-up design strategy.",

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AU - Mitchell, Leslie A

AU - Stracquadanio, Giovanni

AU - Yang, Kun

AU - Dymond, Jessica S

AU - DiCarlo, James E

AU - Lee, Dongwon

AU - Huang, Cheng Lai Victor

AU - Chandrasegaran, Srinivasan

AU - Cai, Yizhi

AU - Boeke, Jef D

AU - Bader, Joel S

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AB - We describe complete design of a synthetic eukaryotic genome, Sc2.0, a highly modified Saccharomyces cerevisiae genome reduced in size by nearly 8%, with 1.1 megabases of the synthetic genome deleted, inserted, or altered. Sc2.0 chromosome design was implemented with BioStudio, an open-source framework developed for eukaryotic genome design, which coordinates design modifications from nucleotide to genome scales and enforces version control to systematically track edits. To achieve complete Sc2.0 genome synthesis, individual synthetic chromosomes built by Sc2.0 Consortium teams around the world will be consolidated into a single strain by "endoreduplication intercross." Chemically synthesized genomes like Sc2.0 are fully customizable and allow experimentalists to ask otherwise intractable questions about chromosome structure, function, and evolution with a bottom-up design strategy.

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Design of a synthetic yeast genome

Abstract

Keywords

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