Designing Zn(II) and Cu(II) derivatives as probes for in vitro fluorescence imaging

Sofia I. Pascu; Philip A. Waghorn; Timothy D. Conry; Helen M. Betts; Jonathan R. Dilworth; Grant C. Churchill; Tzveta Pokrovska; Martin Christlieb; Franklin I. Aigbirhio; John E. Warren

doi:10.1039/b705227h

Designing Zn(II) and Cu(II) derivatives as probes for in vitro fluorescence imaging

Sofia I. Pascu, Philip A. Waghorn, Timothy D. Conry, Helen M. Betts, Jonathan R. Dilworth, Grant C. Churchill, Tzveta Pokrovska, Martin Christlieb, Franklin I. Aigbirhio, John E. Warren

Research output: Contribution to journal › Article › peer-review

Abstract

New M(ii) bis(thiosemicarbazonato) complexes (M = Ni(ii), Cu(ii) and Zn(ii)) featuring allyl groups at the exocyclic nitrogens have been synthesised. The complexes were characterised in solution by spectroscopic methods and their solid state structures determined by single crystal X-ray diffraction using synchrotron radiation. The Zn(ii) complex was found to be intrinsically fluorescent and soluble in biocompatible media. The uptake of this Zn(ii) complex in HeLa, MCF-7 and IGROV cancer cells was monitored by fluorescence microscopies (epi- and confocal fluorescence imaging). The radiolabelling to 64Cu(ii) bis(thiosemicarbazonato) complex was performed cleanly by transmetallation from the corresponding Zn(ii) species using 64Cu(OAc)2. This journal is © The Royal Society of Chemistry.

Original language	English
Pages (from-to)	4988-4997
Number of pages	9
Journal	Dalton Transactions
Issue number	43
DOIs	https://doi.org/10.1039/b705227h
Publication status	Published - 2007

Keywords

BIS(THIOSEMICARBAZONE)
BIS(THIOSEMICARBAZONE)} {RADIOPHARMACEUTICALS
{BIS(THIOSEMICARBAZONE)} {RADIOPHARMACEUTICALS
CANCER-CELLS
{CANCER-CELLS
complexes
{COMPLEXES
COPPER(II)
{COPPER(II)
INTRACELLULAR-DISTRIBUTION
{INTRACELLULAR-DISTRIBUTION
COMPLEXES
ZINC
MICROSCOPY
{MICROSCOPY
PET
{RADIOPHARMACEUTICALS
THIOSEMICARBAZONE
{THIOSEMICARBAZONE
{ZINC

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1039/b705227h

Cite this

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title = "Designing Zn(II) and Cu(II) derivatives as probes for in vitro fluorescence imaging",

abstract = "New M(ii) bis(thiosemicarbazonato) complexes (M = Ni(ii), Cu(ii) and Zn(ii)) featuring allyl groups at the exocyclic nitrogens have been synthesised. The complexes were characterised in solution by spectroscopic methods and their solid state structures determined by single crystal X-ray diffraction using synchrotron radiation. The Zn(ii) complex was found to be intrinsically fluorescent and soluble in biocompatible media. The uptake of this Zn(ii) complex in HeLa, MCF-7 and IGROV cancer cells was monitored by fluorescence microscopies (epi- and confocal fluorescence imaging). The radiolabelling to 64Cu(ii) bis(thiosemicarbazonato) complex was performed cleanly by transmetallation from the corresponding Zn(ii) species using 64Cu(OAc)2. This journal is {\textcopyright} The Royal Society of Chemistry.",

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author = "Pascu, \{Sofia I.\} and Waghorn, \{Philip A.\} and Conry, \{Timothy D.\} and Betts, \{Helen M.\} and Dilworth, \{Jonathan R.\} and Churchill, \{Grant C.\} and Tzveta Pokrovska and Martin Christlieb and Aigbirhio, \{Franklin I.\} and Warren, \{John E.\}",

note = "43",

year = "2007",

doi = "10.1039/b705227h",

language = "English",

pages = "4988--4997",

journal = "Dalton Transactions",

issn = "1477-9234",

publisher = "Royal Society of Chemistry",

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TY - JOUR

T1 - Designing Zn(II) and Cu(II) derivatives as probes for in vitro fluorescence imaging

AU - Pascu, Sofia I.

AU - Waghorn, Philip A.

AU - Conry, Timothy D.

AU - Betts, Helen M.

AU - Dilworth, Jonathan R.

AU - Churchill, Grant C.

AU - Pokrovska, Tzveta

AU - Christlieb, Martin

AU - Aigbirhio, Franklin I.

AU - Warren, John E.

N1 - 43

PY - 2007

Y1 - 2007

N2 - New M(ii) bis(thiosemicarbazonato) complexes (M = Ni(ii), Cu(ii) and Zn(ii)) featuring allyl groups at the exocyclic nitrogens have been synthesised. The complexes were characterised in solution by spectroscopic methods and their solid state structures determined by single crystal X-ray diffraction using synchrotron radiation. The Zn(ii) complex was found to be intrinsically fluorescent and soluble in biocompatible media. The uptake of this Zn(ii) complex in HeLa, MCF-7 and IGROV cancer cells was monitored by fluorescence microscopies (epi- and confocal fluorescence imaging). The radiolabelling to 64Cu(ii) bis(thiosemicarbazonato) complex was performed cleanly by transmetallation from the corresponding Zn(ii) species using 64Cu(OAc)2. This journal is © The Royal Society of Chemistry.

AB - New M(ii) bis(thiosemicarbazonato) complexes (M = Ni(ii), Cu(ii) and Zn(ii)) featuring allyl groups at the exocyclic nitrogens have been synthesised. The complexes were characterised in solution by spectroscopic methods and their solid state structures determined by single crystal X-ray diffraction using synchrotron radiation. The Zn(ii) complex was found to be intrinsically fluorescent and soluble in biocompatible media. The uptake of this Zn(ii) complex in HeLa, MCF-7 and IGROV cancer cells was monitored by fluorescence microscopies (epi- and confocal fluorescence imaging). The radiolabelling to 64Cu(ii) bis(thiosemicarbazonato) complex was performed cleanly by transmetallation from the corresponding Zn(ii) species using 64Cu(OAc)2. This journal is © The Royal Society of Chemistry.

KW - BIS(THIOSEMICARBAZONE)

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UR - https://www.scopus.com/pages/publications/35548997386

U2 - 10.1039/b705227h

DO - 10.1039/b705227h

M3 - Article

SN - 1477-9234

SP - 4988

EP - 4997

JO - Dalton Transactions

JF - Dalton Transactions

IS - 43

ER -

Designing Zn(II) and Cu(II) derivatives as probes for in vitro fluorescence imaging

Abstract

Keywords

UN SDGs

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CCDC 642484: Experimental Crystal Structure Determination

CCDC 642485: Experimental Crystal Structure Determination

CCDC 642486: Experimental Crystal Structure Determination

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Designing Zn(II) and Cu(II) derivatives as probes for in vitro fluorescence imaging

Abstract

Keywords

UN SDGs

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Datasets

CCDC 642484: Experimental Crystal Structure Determination

CCDC 642485: Experimental Crystal Structure Determination

CCDC 642486: Experimental Crystal Structure Determination

Cite this