Detection of genetic instability at HER-2/neu and p53 loci in breast cancer cells sing Comet-FISH

Tirukalikundram S. Kumaravel; Robert G. Bristow

doi:10.1007/s10549-004-5780-0

Detection of genetic instability at HER-2/neu and p53 loci in breast cancer cells sing Comet-FISH

Tirukalikundram S. Kumaravel, Robert G. Bristow^*

^*Corresponding author for this work

Division of Cancer Sciences (L5)

Research output: Contribution to journal › Article › peer-review

Abstract

A proportion of breast cancers acquire genetic alterations at 17q11.2-q12 (HER-2/neu), 20q13.2 (ZNF217 gene) and 17p13.1 (p53). We describe a unique technique (Comet-FISH) in which we documented relative genetic instability at p53 and HER-2/neu gene loci within a panel of malignant breast cancer cell lines (MCF-7; MDA-MB-468 and CRL-2336). Furthermore, Comet-FISH data were consistent with preferential repair of the p53 locus following gentoxic insult and suggest that this assay may be quite useful for the study of genetic instability.

Original language	English
Pages (from-to)	89-93
Number of pages	5
Journal	Breast Cancer Research and Treatment
Volume	91
Issue number	1
DOIs	https://doi.org/10.1007/s10549-004-5780-0
Publication status	Published - May 2005

Keywords

Breast cancer
Breast cancer cell lines
Comet assay
DNA breaks
Fluorescent in situ hybridization (FISH)
Genetic instability
HER-2/neu
p53

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Detection of genetic instability at HER-2/neu and p53 loci in breast cancer cells sing Comet-FISH",

abstract = "A proportion of breast cancers acquire genetic alterations at 17q11.2-q12 (HER-2/neu), 20q13.2 (ZNF217 gene) and 17p13.1 (p53). We describe a unique technique (Comet-FISH) in which we documented relative genetic instability at p53 and HER-2/neu gene loci within a panel of malignant breast cancer cell lines (MCF-7; MDA-MB-468 and CRL-2336). Furthermore, Comet-FISH data were consistent with preferential repair of the p53 locus following gentoxic insult and suggest that this assay may be quite useful for the study of genetic instability.",

keywords = "Breast cancer, Breast cancer cell lines, Comet assay, DNA breaks, Fluorescent in situ hybridization (FISH), Genetic instability, HER-2/neu, p53",

author = "Kumaravel, \{Tirukalikundram S.\} and Bristow, \{Robert G.\}",

year = "2005",

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doi = "10.1007/s10549-004-5780-0",

language = "English",

volume = "91",

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T1 - Detection of genetic instability at HER-2/neu and p53 loci in breast cancer cells sing Comet-FISH

AU - Kumaravel, Tirukalikundram S.

AU - Bristow, Robert G.

PY - 2005/5

Y1 - 2005/5

N2 - A proportion of breast cancers acquire genetic alterations at 17q11.2-q12 (HER-2/neu), 20q13.2 (ZNF217 gene) and 17p13.1 (p53). We describe a unique technique (Comet-FISH) in which we documented relative genetic instability at p53 and HER-2/neu gene loci within a panel of malignant breast cancer cell lines (MCF-7; MDA-MB-468 and CRL-2336). Furthermore, Comet-FISH data were consistent with preferential repair of the p53 locus following gentoxic insult and suggest that this assay may be quite useful for the study of genetic instability.

AB - A proportion of breast cancers acquire genetic alterations at 17q11.2-q12 (HER-2/neu), 20q13.2 (ZNF217 gene) and 17p13.1 (p53). We describe a unique technique (Comet-FISH) in which we documented relative genetic instability at p53 and HER-2/neu gene loci within a panel of malignant breast cancer cell lines (MCF-7; MDA-MB-468 and CRL-2336). Furthermore, Comet-FISH data were consistent with preferential repair of the p53 locus following gentoxic insult and suggest that this assay may be quite useful for the study of genetic instability.

KW - Breast cancer

KW - Breast cancer cell lines

KW - Comet assay

KW - DNA breaks

KW - Fluorescent in situ hybridization (FISH)

KW - Genetic instability

KW - HER-2/neu

KW - p53

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AN - SCOPUS:18844366454

SN - 0167-6806

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JO - Breast Cancer Research and Treatment

JF - Breast Cancer Research and Treatment

IS - 1

ER -

Detection of genetic instability at HER-2/neu and p53 loci in breast cancer cells sing Comet-FISH

Abstract

Keywords

UN SDGs

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