Detection of salt bridges to lysines in solution in barnase.

Mike P Williamson; Andrea M Hounslow; Joe Ford; Kyle Fowler; Max Hebditch; Poul Erik Hansen

doi:10.1039/c3cc45602a

Detection of salt bridges to lysines in solution in barnase.

Mike P Williamson, Andrea M Hounslow, Joe Ford, Kyle Fowler, Max Hebditch, Poul Erik Hansen

The University of Manchester

Research output: Contribution to journal › Article › peer-review

Abstract

We show that salt bridges involving lysines can be detected by deuterium isotope effects on NMR chemical shifts of the sidechain amine. Lys27 in the ribonuclease barnase is salt bridged, and mutation of Arg69 to Lys retains a partially buried salt bridge. The salt bridges are functionally important.

Original language	English
Journal	Chemical communications (Cambridge, England)
Volume	49
Issue number	84
DOIs	https://doi.org/10.1039/c3cc45602a
Publication status	Published - 28 Oct 2013

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title = "Detection of salt bridges to lysines in solution in barnase.",

abstract = "We show that salt bridges involving lysines can be detected by deuterium isotope effects on NMR chemical shifts of the sidechain amine. Lys27 in the ribonuclease barnase is salt bridged, and mutation of Arg69 to Lys retains a partially buried salt bridge. The salt bridges are functionally important.",

author = "Williamson, {Mike P} and Hounslow, {Andrea M} and Joe Ford and Kyle Fowler and Max Hebditch and Hansen, {Poul Erik}",

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T1 - Detection of salt bridges to lysines in solution in barnase.

AU - Williamson, Mike P

AU - Hounslow, Andrea M

AU - Ford, Joe

AU - Fowler, Kyle

AU - Hebditch, Max

AU - Hansen, Poul Erik

PY - 2013/10/28

Y1 - 2013/10/28

N2 - We show that salt bridges involving lysines can be detected by deuterium isotope effects on NMR chemical shifts of the sidechain amine. Lys27 in the ribonuclease barnase is salt bridged, and mutation of Arg69 to Lys retains a partially buried salt bridge. The salt bridges are functionally important.

AB - We show that salt bridges involving lysines can be detected by deuterium isotope effects on NMR chemical shifts of the sidechain amine. Lys27 in the ribonuclease barnase is salt bridged, and mutation of Arg69 to Lys retains a partially buried salt bridge. The salt bridges are functionally important.

U2 - 10.1039/c3cc45602a

DO - 10.1039/c3cc45602a

M3 - Article

C2 - 24030197

SN - 1359-7345

VL - 49

JO - Chemical communications (Cambridge, England)

JF - Chemical communications (Cambridge, England)

IS - 84

ER -

Detection of salt bridges to lysines in solution in barnase.

Abstract

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