Determination of apparent kinetic parameters for competitive product inhibition in packed-bed immobilized enzyme reactors

Ahmet R. Özdural, Deniz Tanyolaç, Ismail H. Boyaci, Mehmet Mutlu, Colin Webb

    Research output: Contribution to journalArticlepeer-review

    Abstract

    In this study, a simple and effective technique for characterizing Michaelis-Menten type kinetics with competitive product inhibition in packed-bed re-circulated immobilized enzyme reactors is presented, where the use of nonlinear regression techniques for multi-parameter estimation are not required. In order to demonstrate the new technique introduced in this work, enzymatic conversion of lactose in a recycling packed-bed reactor is envisaged where β-galactosidase (lactase, EC 3.2.1.23) enzyme is immobilized on a weak base ion exchanger resin (Duolite A 568). For the experimental conditions used in this research, the total competitive inhibition by product (galactose) model is sufficient to represent the lactose hydrolysis kinetics in a packed-bed reactor. © 2002 Elsevier Science B.V. All rights reserved.
    Original languageEnglish
    Pages (from-to)27-36
    Number of pages9
    JournalBiochemical Engineering Journal
    Volume14
    Issue number1
    DOIs
    Publication statusPublished - Apr 2003

    Keywords

    • Competitive inhibition
    • Immobilized enzymes
    • Kinetic parameters
    • Lactose hydrolysis
    • Modeling
    • Packed-bed bioreactors

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