Determination of the epitope of an inhibitory antibody to proliferating cell nuclear antigen

Göran Roos, Yunguan Jiang, Göran Landberg, Niels H. Nielsen, Peng Zhang, Marietta Y W T Lee

    Research output: Contribution to journalArticlepeer-review


    Proliferating cell nuclear antigen (PCNA) is an essential component for the normal processive DNA synthesis by DNA polymerase δ and is also required for DNA excision repair. Human PCNA autoautisera has been shown to inhibit the function of PCNA in vitro, in contrast to induced antibodies. A monoclonal IgG2 PCNA antibody, 74B1, that effectively inhibited DNA replication in vitro was identified. The inhibitory effect was dose dependent and using synthetic overlapping peptides of PCNA the 74B1 epitope was mapped to aa 121-135, a region of the PCNA protein containing the interdomain connector implicated in intermolecular interactions. Interestingly, a neighboring and partly overlapping peptide, aa 111-125, contains an immunodominant region recognized by a number of monoclonal PCNA antibodies with no inhibitory effect on DNA synthesis. The 74B1 antibody is a potentially useful antibody in future studies of PCNA and its interaction in complexes associated with cell cycle progress, DNA replication, and DNA repair.
    Original languageEnglish
    Pages (from-to)208-213
    Number of pages5
    JournalExperimental Cell Research
    Issue number1
    Publication statusPublished - 10 Jul 1996


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