Deuterated carbohydrate probes as 'label-free' substrates for probing nutrient uptake in mycobacteria by nuclear reaction analysis

R. Lowery; M. I. Gibson; R. L. Thompson; E. Fullam

doi:10.1039/c4cc09588j

Deuterated carbohydrate probes as 'label-free' substrates for probing nutrient uptake in mycobacteria by nuclear reaction analysis

R. Lowery, M. I. Gibson, R. L. Thompson, E. Fullam

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Abstract

Understanding and probing small molecule uptake in cells is challenging, requiring sterically large chemical labels, or radioactive isotopes. Here, the uptake of deuterated sugars by Mycobacterium smegmatis, a non-pathogenic model of Mycobacterium tuberculosis, has been investigated using ion-beam (nuclear reaction) analysis demonstrating a new technique for label-free nutrient acquisition measurement.

Original language	English
Pages (from-to)	4838-4841
Number of pages	4
Journal	Chemical Communications
Volume	51
Issue number	23
Early online date	12 Feb 2015
DOIs	https://doi.org/10.1039/c4cc09588j
Publication status	Published - 21 Mar 2015

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title = "Deuterated carbohydrate probes as 'label-free' substrates for probing nutrient uptake in mycobacteria by nuclear reaction analysis",

abstract = "Understanding and probing small molecule uptake in cells is challenging, requiring sterically large chemical labels, or radioactive isotopes. Here, the uptake of deuterated sugars by Mycobacterium smegmatis, a non-pathogenic model of Mycobacterium tuberculosis, has been investigated using ion-beam (nuclear reaction) analysis demonstrating a new technique for label-free nutrient acquisition measurement.",

author = "R. Lowery and Gibson, {M. I.} and Thompson, {R. L.} and E. Fullam",

year = "2015",

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T1 - Deuterated carbohydrate probes as 'label-free' substrates for probing nutrient uptake in mycobacteria by nuclear reaction analysis

AU - Lowery, R.

AU - Gibson, M. I.

AU - Thompson, R. L.

AU - Fullam, E.

PY - 2015/3/21

Y1 - 2015/3/21

N2 - Understanding and probing small molecule uptake in cells is challenging, requiring sterically large chemical labels, or radioactive isotopes. Here, the uptake of deuterated sugars by Mycobacterium smegmatis, a non-pathogenic model of Mycobacterium tuberculosis, has been investigated using ion-beam (nuclear reaction) analysis demonstrating a new technique for label-free nutrient acquisition measurement.

AB - Understanding and probing small molecule uptake in cells is challenging, requiring sterically large chemical labels, or radioactive isotopes. Here, the uptake of deuterated sugars by Mycobacterium smegmatis, a non-pathogenic model of Mycobacterium tuberculosis, has been investigated using ion-beam (nuclear reaction) analysis demonstrating a new technique for label-free nutrient acquisition measurement.

U2 - 10.1039/c4cc09588j

DO - 10.1039/c4cc09588j

M3 - Letter

SN - 1359-7345

VL - 51

SP - 4838

EP - 4841

JO - Chemical Communications

JF - Chemical Communications

IS - 23

ER -

Deuterated carbohydrate probes as 'label-free' substrates for probing nutrient uptake in mycobacteria by nuclear reaction analysis

Abstract

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