Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing aspergillosis.

Deborah Abdul-Ali; Cornelius Clancy; M Hong Nguyen; J Peter Donnelly; Rosemary Barnes; John R Wingard; Michele Sugrue; Barbara D Alexander; Angela M Caliendo; G Marshall Lyon; Lindsey Baden; Francisco Marty; L Joseph Wheat; David Denning; Ming-Hong Nguyen; Cornelius J Clancy; Juergen Loeffler; Stéphane Bretagne; Willem J G Melchers; P Lewis White; Lena Klingspor; Elaine Mc; Manuel Cuenca-Estrella; Thomas F Patterson; Brian Wickes; William R Kirkpatrick; Laura Navjar; Nathan P Wiederhold; Monica Herrera; Scott Filler; Donald C Sheppard; Peter Warn; Mark Wessel; Steven B Kleiboeker; Brent L Seaton

doi:10.1128/JCM.00744-13

Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing aspergillosis.

Deborah Abdul-Ali, Cornelius Clancy, M Hong Nguyen, J Peter Donnelly, Rosemary Barnes, John R Wingard (Collaborator), Michele Sugrue (Collaborator), Barbara D Alexander (Collaborator), Angela M Caliendo (Collaborator), G Marshall Lyon (Collaborator), Lindsey Baden (Collaborator), Francisco Marty (Collaborator), L Joseph Wheat (Collaborator), David Denning (Collaborator), Ming-Hong Nguyen (Collaborator), Cornelius J Clancy (Collaborator), Juergen Loeffler (Collaborator), Stéphane Bretagne (Collaborator), Willem J G Melchers (Collaborator), P Lewis White (Collaborator)Lena Klingspor (Collaborator), Elaine Mc (Collaborator), Manuel Cuenca-Estrella (Collaborator), Thomas F Patterson (Collaborator), Brian Wickes (Collaborator), William R Kirkpatrick (Collaborator), Laura Navjar (Collaborator), Nathan P Wiederhold (Collaborator), Monica Herrera (Collaborator), Scott Filler (Collaborator), Donald C Sheppard (Collaborator), Peter Warn (Collaborator), Mark Wessel (Collaborator), Steven B Kleiboeker (Collaborator), Brent L Seaton (Collaborator)

Research output: Contribution to journal › Article › peer-review

Abstract

Twelve laboratories evaluated candidate material for an Aspergillus DNA calibrator. The DNA material was quantified using limiting-dilution analysis; the mean concentration was determined to be 1.73 × 10(10) units/ml. The calibrator can be used to standardize aspergillosis diagnostic assays which detect and/or quantify nucleic acid.

Original language	English
Journal	Journal of clinical microbiology
Volume	51
Issue number	7
DOIs	https://doi.org/10.1128/JCM.00744-13
Publication status	Published - Jul 2013

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Abdul-Ali, D., Clancy, C., Nguyen, M. H., Donnelly, J. P., Barnes, R., Wingard, J. R., Sugrue, M., Alexander, B. D., Caliendo, A. M., Lyon, G. M., Baden, L., Marty, F., Wheat, L. J., Denning, D., Nguyen, M.-H., Clancy, C. J., Loeffler, J., Bretagne, S., Melchers, W. J. G., ... Seaton, B. L. (2013). Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing aspergillosis. Journal of clinical microbiology, 51(7). https://doi.org/10.1128/JCM.00744-13

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title = "Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing aspergillosis.",

abstract = "Twelve laboratories evaluated candidate material for an Aspergillus DNA calibrator. The DNA material was quantified using limiting-dilution analysis; the mean concentration was determined to be 1.73 × 10(10) units/ml. The calibrator can be used to standardize aspergillosis diagnostic assays which detect and/or quantify nucleic acid.",

author = "Deborah Abdul-Ali and Cornelius Clancy and Nguyen, {M Hong} and Donnelly, {J Peter} and Rosemary Barnes and Wingard, {John R} and Michele Sugrue and Alexander, {Barbara D} and Caliendo, {Angela M} and Lyon, {G Marshall} and Lindsey Baden and Francisco Marty and Wheat, {L Joseph} and David Denning and Ming-Hong Nguyen and Clancy, {Cornelius J} and Juergen Loeffler and St{\'e}phane Bretagne and Melchers, {Willem J G} and White, {P Lewis} and Lena Klingspor and Elaine Mc and Manuel Cuenca-Estrella and Patterson, {Thomas F} and Brian Wickes and Kirkpatrick, {William R} and Laura Navjar and Wiederhold, {Nathan P} and Monica Herrera and Scott Filler and Sheppard, {Donald C} and Peter Warn and Mark Wessel and Kleiboeker, {Steven B} and Seaton, {Brent L}",

note = "266200700023C, PHS HHS, United StatesN01-AI30041, NIAID NIH HHS, United StatesN01-AI70023, NIAID NIH HHS, United States",

year = "2013",

month = jul,

doi = "10.1128/JCM.00744-13",

language = "English",

volume = "51",

journal = "Journal of clinical microbiology",

publisher = "American Society for Microbiology",

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Abdul-Ali, D, Clancy, C, Nguyen, MH, Donnelly, JP, Barnes, R, Wingard, JR, Sugrue, M, Alexander, BD, Caliendo, AM, Lyon, GM, Baden, L, Marty, F, Wheat, LJ, Denning, D, Nguyen, M-H, Clancy, CJ, Loeffler, J, Bretagne, S, Melchers, WJG, White, PL, Klingspor, L, Mc, E, Cuenca-Estrella, M, Patterson, TF, Wickes, B, Kirkpatrick, WR, Navjar, L, Wiederhold, NP, Herrera, M, Filler, S, Sheppard, DC, Warn, P, Wessel, M, Kleiboeker, SB & Seaton, BL 2013, 'Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing aspergillosis.', Journal of clinical microbiology, vol. 51, no. 7. https://doi.org/10.1128/JCM.00744-13

TY - JOUR

T1 - Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing aspergillosis.

AU - Abdul-Ali, Deborah

AU - Clancy, Cornelius

AU - Nguyen, M Hong

AU - Donnelly, J Peter

AU - Barnes, Rosemary

A2 - Wingard, John R

A2 - Sugrue, Michele

A2 - Alexander, Barbara D

A2 - Caliendo, Angela M

A2 - Lyon, G Marshall

A2 - Baden, Lindsey

A2 - Marty, Francisco

A2 - Wheat, L Joseph

A2 - Denning, David

A2 - Nguyen, Ming-Hong

A2 - Clancy, Cornelius J

A2 - Loeffler, Juergen

A2 - Bretagne, Stéphane

A2 - Melchers, Willem J G

A2 - White, P Lewis

A2 - Klingspor, Lena

A2 - Mc, Elaine

A2 - Cuenca-Estrella, Manuel

A2 - Patterson, Thomas F

A2 - Wickes, Brian

A2 - Kirkpatrick, William R

A2 - Navjar, Laura

A2 - Wiederhold, Nathan P

A2 - Herrera, Monica

A2 - Filler, Scott

A2 - Sheppard, Donald C

A2 - Warn, Peter

A2 - Wessel, Mark

A2 - Kleiboeker, Steven B

A2 - Seaton, Brent L

N1 - 266200700023C, PHS HHS, United StatesN01-AI30041, NIAID NIH HHS, United StatesN01-AI70023, NIAID NIH HHS, United States

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AB - Twelve laboratories evaluated candidate material for an Aspergillus DNA calibrator. The DNA material was quantified using limiting-dilution analysis; the mean concentration was determined to be 1.73 × 10(10) units/ml. The calibrator can be used to standardize aspergillosis diagnostic assays which detect and/or quantify nucleic acid.

U2 - 10.1128/JCM.00744-13

DO - 10.1128/JCM.00744-13

M3 - Article

C2 - 23616459

VL - 51

JO - Journal of clinical microbiology

JF - Journal of clinical microbiology

IS - 7

ER -

Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing aspergillosis.

Abstract

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