Development and validation of a rapid HPLC method for the simultaneous determination of triethylenetetramine and its two main metabolites in human serum

Asma Othman; Jun Lu; Tracey Sunderland; Garth J.S. Cooper

doi:10.1016/j.jchromb.2007.10.006

Development and validation of a rapid HPLC method for the simultaneous determination of triethylenetetramine and its two main metabolites in human serum

Asma Othman, Jun Lu, Tracey Sunderland, Garth J.S. Cooper

Division of Cardiovascular Sciences (L5)

University of Auckland

Research output: Contribution to journal › Article › peer-review

Abstract

A validated method for the determination of triethylenetetramine, a selective copper-chelator currently undergoing clinical trials for the treatment of diabetic heart failure, and its two major metabolites, N1-acetyltriethylenetetramine and N1,N10-diacetyltriethylenetetramine in human serum using HPLC is reported. The method used 9-flouorenylmethylchloroformate chloride to label all three analytes. The fluorescence labeled analytes were then separated chromatographically using a reversed phase C18 column under a gradient elution program and detected spectrofluorometrically at 317 nm with excitation at 263 nm. Application of the method is demonstrated by pharmacokinetic measurement in one healthy volunteer taking the drug orally. © 2007 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	42-48
Number of pages	6
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	860
Issue number	1
DOIs	https://doi.org/10.1016/j.jchromb.2007.10.006
Publication status	Published - 1 Dec 2007

Keywords

Diabetic heart failure
FMOC
HPLC
LC-MS
TETA

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jchromb.2007.10.006

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title = "Development and validation of a rapid HPLC method for the simultaneous determination of triethylenetetramine and its two main metabolites in human serum",

abstract = "A validated method for the determination of triethylenetetramine, a selective copper-chelator currently undergoing clinical trials for the treatment of diabetic heart failure, and its two major metabolites, N1-acetyltriethylenetetramine and N1,N10-diacetyltriethylenetetramine in human serum using HPLC is reported. The method used 9-flouorenylmethylchloroformate chloride to label all three analytes. The fluorescence labeled analytes were then separated chromatographically using a reversed phase C18 column under a gradient elution program and detected spectrofluorometrically at 317 nm with excitation at 263 nm. Application of the method is demonstrated by pharmacokinetic measurement in one healthy volunteer taking the drug orally. {\textcopyright} 2007 Elsevier B.V. All rights reserved.",

keywords = "Diabetic heart failure, FMOC, HPLC, LC-MS, TETA",

author = "Asma Othman and Jun Lu and Tracey Sunderland and Cooper, {Garth J.S.}",

year = "2007",

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language = "English",

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journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",

issn = "1570-0232",

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Othman, A, Lu, J, Sunderland, T & Cooper, GJS 2007, 'Development and validation of a rapid HPLC method for the simultaneous determination of triethylenetetramine and its two main metabolites in human serum', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 860, no. 1, pp. 42-48. https://doi.org/10.1016/j.jchromb.2007.10.006

Development and validation of a rapid HPLC method for the simultaneous determination of triethylenetetramine and its two main metabolites in human serum. / Othman, Asma; Lu, Jun; Sunderland, Tracey et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 860, No. 1, 01.12.2007, p. 42-48.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Development and validation of a rapid HPLC method for the simultaneous determination of triethylenetetramine and its two main metabolites in human serum

AU - Othman, Asma

AU - Lu, Jun

AU - Sunderland, Tracey

AU - Cooper, Garth J.S.

PY - 2007/12/1

Y1 - 2007/12/1

N2 - A validated method for the determination of triethylenetetramine, a selective copper-chelator currently undergoing clinical trials for the treatment of diabetic heart failure, and its two major metabolites, N1-acetyltriethylenetetramine and N1,N10-diacetyltriethylenetetramine in human serum using HPLC is reported. The method used 9-flouorenylmethylchloroformate chloride to label all three analytes. The fluorescence labeled analytes were then separated chromatographically using a reversed phase C18 column under a gradient elution program and detected spectrofluorometrically at 317 nm with excitation at 263 nm. Application of the method is demonstrated by pharmacokinetic measurement in one healthy volunteer taking the drug orally. © 2007 Elsevier B.V. All rights reserved.

AB - A validated method for the determination of triethylenetetramine, a selective copper-chelator currently undergoing clinical trials for the treatment of diabetic heart failure, and its two major metabolites, N1-acetyltriethylenetetramine and N1,N10-diacetyltriethylenetetramine in human serum using HPLC is reported. The method used 9-flouorenylmethylchloroformate chloride to label all three analytes. The fluorescence labeled analytes were then separated chromatographically using a reversed phase C18 column under a gradient elution program and detected spectrofluorometrically at 317 nm with excitation at 263 nm. Application of the method is demonstrated by pharmacokinetic measurement in one healthy volunteer taking the drug orally. © 2007 Elsevier B.V. All rights reserved.

KW - Diabetic heart failure

KW - FMOC

KW - HPLC

KW - LC-MS

KW - TETA

U2 - 10.1016/j.jchromb.2007.10.006

DO - 10.1016/j.jchromb.2007.10.006

M3 - Article

C2 - 17980682

SN - 1570-0232

VL - 860

SP - 42

EP - 48

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

IS - 1

ER -

Development and validation of a rapid HPLC method for the simultaneous determination of triethylenetetramine and its two main metabolites in human serum

Abstract

Keywords

UN SDGs

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