Development of a green fluorescent protein reporter for a yeast genotoxicity biosensor

N. Billinton; M. G. Barker; C. E. Michel; A. W. Knight; W. D. Heyer; N. J. Goddard; P. R. Fielden; R. M. Walmsley

doi:10.1016/S0956-5663(98)00049-9

Development of a green fluorescent protein reporter for a yeast genotoxicity biosensor

N. Billinton, M. G. Barker, C. E. Michel, A. W. Knight, W. D. Heyer, N. J. Goddard, P. R. Fielden, R. M. Walmsley

Research output: Chapter in Book/Conference proceeding › Conference contribution

Abstract

A reporter system, constructed for a laboratory screen for new genes involved in DNA repair in the brewer's yeast Saccharomyces cerevisiae, has been developed for use in a genotoxicity biosensor. The strain produces green fluorescent protein (yEGFP) when DNA damage has occurred. yEGFP is codon optimised for yeasts. The reporter does not respond to chemicals which delay mitosis, and responds appropriately to the genetic regulation of DNA repair. Data is presented which demonstrate strain improvements appropriate to biosensor technology: improved signal to noise ratio, ease of data collection and uncomplicated material handling.

Original language	English
Title of host publication	Biosensors and Bioelectronics\|Biosens. Bioelectron.
Editors	A.P.F. Turner
Pages	831-838
Number of pages	7
Volume	13
DOIs	https://doi.org/10.1016/S0956-5663(98)00049-9
Publication status	Published - Oct 1998
Event	Proceedings of the 1998 5th World Congress on Biosensors - Berlin, Ger Duration: 1 Oct 1998 → …

Conference

Conference	Proceedings of the 1998 5th World Congress on Biosensors
City	Berlin, Ger
Period	1/10/98 → …

Keywords

Biosensor
DNA repair
Fluorescence
Genotoxicity
GFP
RAD54
Saccharomyces cerevisiae

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10.1016/S0956-5663(98)00049-9

Cite this

@inproceedings{e59e8104b00c4be2bf2c146a0c3249c9,

title = "Development of a green fluorescent protein reporter for a yeast genotoxicity biosensor",

abstract = "A reporter system, constructed for a laboratory screen for new genes involved in DNA repair in the brewer's yeast Saccharomyces cerevisiae, has been developed for use in a genotoxicity biosensor. The strain produces green fluorescent protein (yEGFP) when DNA damage has occurred. yEGFP is codon optimised for yeasts. The reporter does not respond to chemicals which delay mitosis, and responds appropriately to the genetic regulation of DNA repair. Data is presented which demonstrate strain improvements appropriate to biosensor technology: improved signal to noise ratio, ease of data collection and uncomplicated material handling.",

keywords = "Biosensor, DNA repair, Fluorescence, Genotoxicity, GFP, RAD54, Saccharomyces cerevisiae",

author = "N. Billinton and Barker, {M. G.} and Michel, {C. E.} and Knight, {A. W.} and Heyer, {W. D.} and Goddard, {N. J.} and Fielden, {P. R.} and Walmsley, {R. M.}",

year = "1998",

month = oct,

doi = "10.1016/S0956-5663(98)00049-9",

language = "English",

volume = "13",

pages = "831--838",

editor = "A.P.F. Turner",

booktitle = "Biosensors and Bioelectronics|Biosens. Bioelectron.",

note = "Proceedings of the 1998 5th World Congress on Biosensors ; Conference date: 01-10-1998",

}

Billinton, N, Barker, MG, Michel, CE, Knight, AW, Heyer, WD, Goddard, NJ, Fielden, PR & Walmsley, RM 1998, Development of a green fluorescent protein reporter for a yeast genotoxicity biosensor. in APF Turner (ed.), Biosensors and Bioelectronics|Biosens. Bioelectron.. vol. 13, pp. 831-838, Proceedings of the 1998 5th World Congress on Biosensors, Berlin, Ger, 1/10/98. https://doi.org/10.1016/S0956-5663(98)00049-9

TY - GEN

T1 - Development of a green fluorescent protein reporter for a yeast genotoxicity biosensor

AU - Billinton, N.

AU - Barker, M. G.

AU - Michel, C. E.

AU - Knight, A. W.

AU - Heyer, W. D.

AU - Goddard, N. J.

AU - Fielden, P. R.

AU - Walmsley, R. M.

PY - 1998/10

Y1 - 1998/10

N2 - A reporter system, constructed for a laboratory screen for new genes involved in DNA repair in the brewer's yeast Saccharomyces cerevisiae, has been developed for use in a genotoxicity biosensor. The strain produces green fluorescent protein (yEGFP) when DNA damage has occurred. yEGFP is codon optimised for yeasts. The reporter does not respond to chemicals which delay mitosis, and responds appropriately to the genetic regulation of DNA repair. Data is presented which demonstrate strain improvements appropriate to biosensor technology: improved signal to noise ratio, ease of data collection and uncomplicated material handling.

AB - A reporter system, constructed for a laboratory screen for new genes involved in DNA repair in the brewer's yeast Saccharomyces cerevisiae, has been developed for use in a genotoxicity biosensor. The strain produces green fluorescent protein (yEGFP) when DNA damage has occurred. yEGFP is codon optimised for yeasts. The reporter does not respond to chemicals which delay mitosis, and responds appropriately to the genetic regulation of DNA repair. Data is presented which demonstrate strain improvements appropriate to biosensor technology: improved signal to noise ratio, ease of data collection and uncomplicated material handling.

KW - Biosensor

KW - DNA repair

KW - Fluorescence

KW - Genotoxicity

KW - GFP

KW - RAD54

KW - Saccharomyces cerevisiae

U2 - 10.1016/S0956-5663(98)00049-9

DO - 10.1016/S0956-5663(98)00049-9

M3 - Conference contribution

VL - 13

SP - 831

EP - 838

BT - Biosensors and Bioelectronics|Biosens. Bioelectron.

A2 - Turner, A.P.F.

T2 - Proceedings of the 1998 5th World Congress on Biosensors

Y2 - 1 October 1998

ER -

Development of a green fluorescent protein reporter for a yeast genotoxicity biosensor

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Keywords

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