Developmental fate determination and marker discovery in hematopoietic stem cell biology using proteomic fingerprinting

Elaine Spooncer, Nathalie Brouard, Susie K. Nilsson, Brenda Williams, Mira C. Liu, Richard D. Unwin, David Blinco, Ewa Jaworska, Paul J. Simmons, Anthony D. Whetton

    Research output: Contribution to journalArticlepeer-review

    Abstract

    In hematopoiesis, co-expression of Sca-1 and c-Kit defines cells (LS+ K) with long term reconstituting potential. In contrast, poorly characterized LS-K cells fail to reconstitute lethally irradiated recipients. Relative quantification mass spectrometry and transcriptional profiling were used to characterize LS+K and LS-K cells. This approach yielded date on >1200 proteins. Only 32% of protein changes correlated to mRNA modulation demonstrating post-translational protein regulation in early hematopoietic development. LS+K cells had lower expression of protein synthesis proteins but did express proteins associated with mature cell function. Major increases in erythroid development proteins were observed in LS-K cells; based on this assessment of erythroid potential we showed them to be principally erythroid progenitors, demonstrating effective use of discovery proteomics for definition of primitive cells. © 2008 by The American Society for Biochemistry and Molecular Biology, Inc.
    Original languageEnglish
    Pages (from-to)573-581
    Number of pages8
    JournalMolecular and Cellular Proteomics
    Volume7
    Issue number3
    DOIs
    Publication statusPublished - Mar 2008

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