Diagnosis of herpes encephalitis via Southern blotting of cerebrospinal fluid DNA amplified by polymerase chain reaction

P E Klapper; G M Cleator; C Dennett; A G Lewis

doi:10.1002/jmv.1890320413

Diagnosis of herpes encephalitis via Southern blotting of cerebrospinal fluid DNA amplified by polymerase chain reaction

P E Klapper, G M Cleator, C Dennett, A G Lewis

Research output: Contribution to journal › Article › peer-review

Abstract

Herpes simplex virus thymidine kinase gene specific polymerase chain reaction (PCR) amplification of DNA extracted from lumbar cerebrospinal fluid (CSF) and Southern blotting (SB) were evaluated as a method for the diagnosis of herpes simplex encephalitis (HSE). Positive PCR-SB results were obtained with CSF samples from 9 of 10 patients (11 of 12 CSF specimens) with proven herpes encephalitis as early as 2 days after onset of neurological illness. Our data support the suggestion that PCR techniques may provide a clinically relevant "non-invasive" method for the diagnosis of HSE.

Original language	English
Pages (from-to)	261-4
Number of pages	4
Journal	Journal Of Medical Virology
Volume	32
Issue number	4
DOIs	https://doi.org/10.1002/jmv.1890320413
Publication status	Published - 1 Dec 1990

Keywords

Base Sequence
Blotting, Southern
DNA, Viral/cerebrospinal fluid
Encephalitis/complications
Herpes Simplex/complications
Humans
Molecular Sequence Data
Polymerase Chain Reaction
Simplexvirus/enzymology
Thymidine Kinase/genetics

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10.1002/jmv.1890320413

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title = "Diagnosis of herpes encephalitis via Southern blotting of cerebrospinal fluid DNA amplified by polymerase chain reaction",

abstract = "Herpes simplex virus thymidine kinase gene specific polymerase chain reaction (PCR) amplification of DNA extracted from lumbar cerebrospinal fluid (CSF) and Southern blotting (SB) were evaluated as a method for the diagnosis of herpes simplex encephalitis (HSE). Positive PCR-SB results were obtained with CSF samples from 9 of 10 patients (11 of 12 CSF specimens) with proven herpes encephalitis as early as 2 days after onset of neurological illness. Our data support the suggestion that PCR techniques may provide a clinically relevant {"}non-invasive{"} method for the diagnosis of HSE.",

keywords = "Base Sequence, Blotting, Southern, DNA, Viral/cerebrospinal fluid, Encephalitis/complications, Herpes Simplex/complications, Humans, Molecular Sequence Data, Polymerase Chain Reaction, Simplexvirus/enzymology, Thymidine Kinase/genetics",

author = "Klapper, {P E} and Cleator, {G M} and C Dennett and Lewis, {A G}",

year = "1990",

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doi = "10.1002/jmv.1890320413",

language = "English",

volume = "32",

pages = "261--4",

journal = "Journal Of Medical Virology",

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TY - JOUR

T1 - Diagnosis of herpes encephalitis via Southern blotting of cerebrospinal fluid DNA amplified by polymerase chain reaction

AU - Klapper, P E

AU - Cleator, G M

AU - Dennett, C

AU - Lewis, A G

PY - 1990/12/1

Y1 - 1990/12/1

N2 - Herpes simplex virus thymidine kinase gene specific polymerase chain reaction (PCR) amplification of DNA extracted from lumbar cerebrospinal fluid (CSF) and Southern blotting (SB) were evaluated as a method for the diagnosis of herpes simplex encephalitis (HSE). Positive PCR-SB results were obtained with CSF samples from 9 of 10 patients (11 of 12 CSF specimens) with proven herpes encephalitis as early as 2 days after onset of neurological illness. Our data support the suggestion that PCR techniques may provide a clinically relevant "non-invasive" method for the diagnosis of HSE.

AB - Herpes simplex virus thymidine kinase gene specific polymerase chain reaction (PCR) amplification of DNA extracted from lumbar cerebrospinal fluid (CSF) and Southern blotting (SB) were evaluated as a method for the diagnosis of herpes simplex encephalitis (HSE). Positive PCR-SB results were obtained with CSF samples from 9 of 10 patients (11 of 12 CSF specimens) with proven herpes encephalitis as early as 2 days after onset of neurological illness. Our data support the suggestion that PCR techniques may provide a clinically relevant "non-invasive" method for the diagnosis of HSE.

KW - Base Sequence

KW - Blotting, Southern

KW - DNA, Viral/cerebrospinal fluid

KW - Encephalitis/complications

KW - Herpes Simplex/complications

KW - Humans

KW - Molecular Sequence Data

KW - Polymerase Chain Reaction

KW - Simplexvirus/enzymology

KW - Thymidine Kinase/genetics

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DO - 10.1002/jmv.1890320413

M3 - Article

C2 - 1964477

SN - 0146-6615

VL - 32

SP - 261

EP - 264

JO - Journal Of Medical Virology

JF - Journal Of Medical Virology

IS - 4

ER -

Diagnosis of herpes encephalitis via Southern blotting of cerebrospinal fluid DNA amplified by polymerase chain reaction

Abstract

Keywords

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