Dicer-dependent miRNAs provide an endogenous restraint on cytotrophoblast proliferation

K. Forbes; F. Farrokhnia; J. D. Aplin; M. Westwood

doi:10.1016/j.placenta.2012.03.006

Dicer-dependent miRNAs provide an endogenous restraint on cytotrophoblast proliferation

K. Forbes, F. Farrokhnia, J. D. Aplin, M. Westwood

Research output: Contribution to journal › Article › peer-review

Abstract

Mature microRNAs (miRNAs) are processed from non-functional (pre)-miRNAs by the enzyme Dicer. In this study, manipulation of Dicer level was used to explore the influence of miRNAs on cytotrophoblast proliferation in human placenta. Immunohistochemistry (IHC) showed Dicer in cytotrophoblast, but not in terminally differentiated syncytiotrophoblast. siRNA-mediated knockdown of Dicer was used to effect a global reduction in miRNA in first trimester placental explants, as a result of which cytotrophoblast proliferation was significantly enhanced. QPCR and IHC analysis following Dicer knockdown revealed that the expression of two nodal pro-mitogenic signalling molecules expressed within cytotrophoblast, ERK and SHP-2, was significantly enhanced. Studies are now required to identify individual miRNAs involved in regulating trophoblast proliferation. © 2012 Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	581-585
Number of pages	4
Journal	Placenta
Volume	33
Issue number	7
DOIs	https://doi.org/10.1016/j.placenta.2012.03.006
Publication status	Published - Jul 2012

Keywords

ERK
IGF
miRNA
SHP-2
siRNA

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10.1016/j.placenta.2012.03.006

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title = "Dicer-dependent miRNAs provide an endogenous restraint on cytotrophoblast proliferation",

abstract = "Mature microRNAs (miRNAs) are processed from non-functional (pre)-miRNAs by the enzyme Dicer. In this study, manipulation of Dicer level was used to explore the influence of miRNAs on cytotrophoblast proliferation in human placenta. Immunohistochemistry (IHC) showed Dicer in cytotrophoblast, but not in terminally differentiated syncytiotrophoblast. siRNA-mediated knockdown of Dicer was used to effect a global reduction in miRNA in first trimester placental explants, as a result of which cytotrophoblast proliferation was significantly enhanced. QPCR and IHC analysis following Dicer knockdown revealed that the expression of two nodal pro-mitogenic signalling molecules expressed within cytotrophoblast, ERK and SHP-2, was significantly enhanced. Studies are now required to identify individual miRNAs involved in regulating trophoblast proliferation. {\textcopyright} 2012 Elsevier Ltd. All rights reserved.",

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author = "K. Forbes and F. Farrokhnia and Aplin, {J. D.} and M. Westwood",

year = "2012",

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language = "English",

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TY - JOUR

T1 - Dicer-dependent miRNAs provide an endogenous restraint on cytotrophoblast proliferation

AU - Forbes, K.

AU - Farrokhnia, F.

AU - Aplin, J. D.

AU - Westwood, M.

PY - 2012/7

Y1 - 2012/7

N2 - Mature microRNAs (miRNAs) are processed from non-functional (pre)-miRNAs by the enzyme Dicer. In this study, manipulation of Dicer level was used to explore the influence of miRNAs on cytotrophoblast proliferation in human placenta. Immunohistochemistry (IHC) showed Dicer in cytotrophoblast, but not in terminally differentiated syncytiotrophoblast. siRNA-mediated knockdown of Dicer was used to effect a global reduction in miRNA in first trimester placental explants, as a result of which cytotrophoblast proliferation was significantly enhanced. QPCR and IHC analysis following Dicer knockdown revealed that the expression of two nodal pro-mitogenic signalling molecules expressed within cytotrophoblast, ERK and SHP-2, was significantly enhanced. Studies are now required to identify individual miRNAs involved in regulating trophoblast proliferation. © 2012 Elsevier Ltd. All rights reserved.

AB - Mature microRNAs (miRNAs) are processed from non-functional (pre)-miRNAs by the enzyme Dicer. In this study, manipulation of Dicer level was used to explore the influence of miRNAs on cytotrophoblast proliferation in human placenta. Immunohistochemistry (IHC) showed Dicer in cytotrophoblast, but not in terminally differentiated syncytiotrophoblast. siRNA-mediated knockdown of Dicer was used to effect a global reduction in miRNA in first trimester placental explants, as a result of which cytotrophoblast proliferation was significantly enhanced. QPCR and IHC analysis following Dicer knockdown revealed that the expression of two nodal pro-mitogenic signalling molecules expressed within cytotrophoblast, ERK and SHP-2, was significantly enhanced. Studies are now required to identify individual miRNAs involved in regulating trophoblast proliferation. © 2012 Elsevier Ltd. All rights reserved.

KW - ERK

KW - IGF

KW - miRNA

KW - SHP-2

KW - siRNA

U2 - 10.1016/j.placenta.2012.03.006

DO - 10.1016/j.placenta.2012.03.006

M3 - Article

C2 - 22516645

SN - 0143-4004

VL - 33

SP - 581

EP - 585

JO - Placenta

JF - Placenta

IS - 7

ER -

Dicer-dependent miRNAs provide an endogenous restraint on cytotrophoblast proliferation

Abstract

Keywords

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