Diet-induced obesity, exogenous leptin-, and MADB106 tumor cell challenge affect tissue leukocyte distribution and serum levels of cytokines in F344 rats

Patrick Behrendt, Tobias Buchenauer, Rüdiger Horn, Georg Brabant, Roland Jacobs, Felix Bode, Michael Stephan, Heike Nave

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The adipocyte-derived catabolic protein leptin alters cell-mediated immunity and cytokine crosstalk. This may provide new insights into the altered immune response, seen in obese individuals. Therefore, we determined the tissue distribution of immune cells in diet-induced obese (dio) and normal weight F344 rats challenged with MADB106 tumor cells or leptin. Immune cell distribution in blood (by FACS analysis) and tissues (NK cells in spleen and liver, immunohistologically) as well as pro-inflammatory cytokines (IL-6, TNF-α; by flow cytometry) were investigated in 28 normal weight and 28 dio rats (n = 4-6/group). Pro-inflammatory cytokines were increased 3-fold for IL-6 and 7-fold for TNF-α in obese animals. Higher numbers of blood monocytes and NK cells were found in obese as compared to normal weight animals. In dio rats challenged with leptin and MADB106 tumor cells, monocyte numbers were decreased as compared to the obese control animals. Immunohistochemistry revealed an altered NK cell distribution in a compartment-, treatment-, and bodyweight-specific manner. In conclusion, our data reveal a distinct distribution pattern of monocytes and NK cells in dio rats as compared to normal weight littermates and an additional modulatory effect of a leptin- and MADB106 tumor cell challenge. © 2010 Springer Science+Business Media, LLC.
    Original languageEnglish
    Pages (from-to)104-112
    Number of pages8
    JournalEndocrine
    Volume38
    Issue number1
    DOIs
    Publication statusPublished - Aug 2010

    Keywords

    • Chronic inflammation
    • IL-6
    • Leptin
    • NK cells
    • Obesity
    • TNF-α

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