Different oxygen treatment pressures alter inflammatory gene expression in human endothelial cells

Alexandra C. Kendall, Jacqueline L. Whatmore, Lorna W. Harries, Paul G. Winyard, Paul Eggleton, Gary R. Smerdon

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Hyperbaric oxygen has proven to be a useful treatment for chronic wounds. However, therapeutic conditions vary between treatment centers, and we wished to investigate the effects of different treatment pressures on cells under inflammatory conditions. Endothelial cells were exposed to a chronic wound model comprising hypoxia (2% O2 at 1 atmosphere absolute (atm abs); PO2 ∼ 2 kPa) in the presence of 0.5 μg/ml lipopolysaccharide and 1 ng/ml TNF-α for 24 hours, then treated with normobaric oxygen (NBO2; 95%O2/5%CO2 at 1.0 atm abs; PO 2 ∼ 96.3 kPa), hyperbaric oxygen (HBO2) at 1.5 atm abs (1.5HBO2; 96.7%O2/3.3%CO2 at 1.5 atm abs; PO2 ∼ 147 kPa), and HBO2 at 2.4 atm abs (2.4HBO 2; 97.9%O2/2.1%CO2 at 2.4 atm abs; PO 2 ∼ 238 kPa). The mRNA expression of 92 inflammatory genes was then analyzed, and we identified changes in genes involved in adhesion molecule expression, angiogenesis and tissue remodeling, intracellular signaling, and cellular oxygen responses and redox signaling. We noted differences in expression between different treatment pressures, highlighting the need for further research into the use of different therapeutic protocols in the treatment of inflammatory conditions such as chronic wounds. Copyright © 2013 Undersea & Hyperbaric Medical Society, Inc.
    Original languageEnglish
    Pages (from-to)115-123
    Number of pages8
    JournalUndersea and Hyperbaric Medicine
    Volume40
    Issue number2
    Publication statusPublished - Mar 2013

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