Differential expression of adenylyl cyclase subtypes in human cardiovascular system

T. Wang, M. J. Brown

    Research output: Contribution to journalArticlepeer-review

    Abstract

    In human myocardium, β 1-adrenoceptor stimulation achieves maximal inotropic response but less than 50% of maximal adenylyl cyclase activation, whereas the reverse is true of the β 2-adrenoceptor. Four types of adenylyl cyclase, type IV-VII, have been described in mammalian heart, but their expression and relative distribution in human heart and blood vessels is not known. We found that type IV, V, VI and VII adenylyl cyclases were all expressed in cardiomyocytes. Whereas types IV and VII RNA were more abundant in extra-cardiac than cardiac tissues, both absolute and relative expression of type VI was greatest in heart, and lower in tissues lacking a β 1-adrenoceptor. Type V expression was virtually confined to atrium. In situ mRNA hybridisation showed that the β 1- adrenoceptor co-localised with type VI adenylyl cyclase but not other subtypes in juxtoglomerular cells of human kidney. The tissue specific expression of these adenylyl cyclase subtypes may favour its coupling to corresponding receptors expressed in the given tissue type. © 2004 Elsevier Ireland Ltd. All rights reserved.
    Original languageEnglish
    Pages (from-to)55-62
    Number of pages7
    JournalMolecular and Cellular Endocrinology
    Volume223
    Issue number1-2
    DOIs
    Publication statusPublished - 31 Aug 2004

    Keywords

    • β-Adrenergic receptor
    • Adenylyl cyclase
    • In situ mRNA hybridisation
    • mRNA expression
    • Signal transduction
    • TaqMan PCR

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