Differential innate immune responses of a living skin equivalent model colonized by staphylococcus epidermidis or staphylococcus aureus

Diana B. Holland, Richard A. Bojar, Mark D. Farrar, Keith T. Holland

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Staphylococcus epidermidis is a commensal on skin, whereas Staphylococcus aureus is a transient pathogen. The aim was to determine whether the skin's innate defence systems responded differently to these microorganisms. Differential gene expression of a human skin equivalent (SE) model was assessed by microarray technology, in response to colonization by S. epidermidis or S. aureus. Only a small number of transcripts were significantly (P2-fold on SEs colonized with S. epidermidis compared with controls (no colonization). Expression of one innate defence gene, pentraxin 3 (PTX3), was upregulated, while psoriasin, S100A12, S100A15, β defensin 4, β defensin 3, lipocalin 2 and peptidoglycan recognition protein 2 were downregulated. In contrast, large numbers of transcripts were significantly increased (480) or decreased (397) with gene expression changes of >2-fold on SEs colonized with S. aureus compared with controls. There was upregulation in gene expression of many skin defence factors including Toll-like receptor 2, β defensin 4, properdin, PTX3, proinflammatory cytokines tumour necrosis factor-α, IL-1α, IL-1β, IL-17C, IL-20, IL-23A and chemokines IL-8, CCL4, CCL5, CCL20 and CCL27. These differences may partly explain why S. epidermidis is a normal skin resident and S. aureus is not. © 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
    Original languageEnglish
    Pages (from-to)149-155
    Number of pages6
    JournalFEMS microbiology letters
    Volume290
    Issue number2
    DOIs
    Publication statusPublished - Jan 2009

    Keywords

    • Innate immunity
    • Microarray
    • Skin equivalent model
    • Staphylococcus aureus
    • Staphylococcus epidermidis

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